IADR Abstract Archives
Activity against multispecies biofilms and chemical and mechanical properties of nanomaterial-modified polymers
Objectives: This study proposed methods for the incorporation of nanostructured silver vanadate (AgVO3) into a dental acrylic resin and evaluated the chemical composition, efficacy against Staphylococcus aureus, Candida albicans and Pseudomonas aeruginosa in multispecies biofilms and its mechanical performance.
Methods: The AgVO3 was incorporated into the resin in fractions of 0-5%, by two methodologies: (I) vacuum spatulation and (II) polymer film technique. The characterization was performed by scanning electron microscopy and microanalysis (SEM/EDS). The antibiofilm efficacy was evaluated by counting colony forming units and fluorescence microscopy, and the mechanical performance was evaluated by flexural strength, impact and surface hardness tests. The data were analyzed by 2-factor ANOVA with multiple comparisons with Bonferroni and Kruskal-Wallis adjustments followed by Dunn's post-test (α=0.05).
Results: There were differences in the dispersion pattern promoted by the methods and in the chemical composition. Higher AgVO3 concentrations are effective against S. aureus (p<0.001), with no difference between incorporation methods (p=0.194). The addition of AgVO3 reduced the flexural strength and impact strength of the resins (p<0.001). The polymer film technique promoted better flexural strength (p<0.001). The vacuum spatulation technique promoted greater surface hardness (p<0.001), especially with the addition of 5% of AgVO3 (p<0.05).
Conclusions: It is concluded that the multispecies biofilms are affected depending on the levels of AgVO3 and the nature of the microbial species. The concentration of the nanomaterial and incorporation technique influences the mechanical properties of the acrylic resin, however, it may be clinically acceptable.
Methods: The AgVO3 was incorporated into the resin in fractions of 0-5%, by two methodologies: (I) vacuum spatulation and (II) polymer film technique. The characterization was performed by scanning electron microscopy and microanalysis (SEM/EDS). The antibiofilm efficacy was evaluated by counting colony forming units and fluorescence microscopy, and the mechanical performance was evaluated by flexural strength, impact and surface hardness tests. The data were analyzed by 2-factor ANOVA with multiple comparisons with Bonferroni and Kruskal-Wallis adjustments followed by Dunn's post-test (α=0.05).
Results: There were differences in the dispersion pattern promoted by the methods and in the chemical composition. Higher AgVO3 concentrations are effective against S. aureus (p<0.001), with no difference between incorporation methods (p=0.194). The addition of AgVO3 reduced the flexural strength and impact strength of the resins (p<0.001). The polymer film technique promoted better flexural strength (p<0.001). The vacuum spatulation technique promoted greater surface hardness (p<0.001), especially with the addition of 5% of AgVO3 (p<0.05).
Conclusions: It is concluded that the multispecies biofilms are affected depending on the levels of AgVO3 and the nature of the microbial species. The concentration of the nanomaterial and incorporation technique influences the mechanical properties of the acrylic resin, however, it may be clinically acceptable.