IADR Abstract Archives
Asymptomatic Apical Periodontitis Macrophages Exhibit an M1 Profile
Objectives: To determine the polarization profile of macrophages in Asymptomatic Apical Periodontitis (AAP) and healthy controls.
Methods: AAP (n = 6) and healthy controls (n = 7), were obtained from chronic apical periodontitis and healthy periodontal ligament (HPL) with indication of tooth extraction, respectively. Inclusion criteria were teeth with negative response to the sensitivity test and percussion test and AL ≥3 mm at radiographic examination. Individuals with systemic disease or individuals with previous use of antibiotics or anti-inflammatory steroidal drugs during a period of 3 months were excluded. Samples were enzymatically digested and then labeled with anti-CD14, anti-CD64, anti-CD80, anti-CD163 and anti-CD206 antibodies. Finally, samples were fixed with 2% paraformaldehyde and characterized by flow cytometry. Analysis was performed with Student's T test.
Results: Higher percentage of CD14+ cells were observed over the total number of cells in AAP over HPL (53.2% and 10.8% respectively, p <0.005). Also, higher percentage of CD64+ CD80+ cells were identified over the total number of CD14+ cells in AAP over HPL (17.5% and 38.92% respectively, p <0.05), whereas no significant elevation of the percentage of CD163+ CD206+ cells was observed over the total number of CD14+ cells.
Conclusions: The results support an increase in the population of CD14+ cells and M1 over M2 macrophages in AAP versus HPL. This suggests a pro inflammatory profile and the progression of the AAP lesion.
Methods: AAP (n = 6) and healthy controls (n = 7), were obtained from chronic apical periodontitis and healthy periodontal ligament (HPL) with indication of tooth extraction, respectively. Inclusion criteria were teeth with negative response to the sensitivity test and percussion test and AL ≥3 mm at radiographic examination. Individuals with systemic disease or individuals with previous use of antibiotics or anti-inflammatory steroidal drugs during a period of 3 months were excluded. Samples were enzymatically digested and then labeled with anti-CD14, anti-CD64, anti-CD80, anti-CD163 and anti-CD206 antibodies. Finally, samples were fixed with 2% paraformaldehyde and characterized by flow cytometry. Analysis was performed with Student's T test.
Results: Higher percentage of CD14+ cells were observed over the total number of cells in AAP over HPL (53.2% and 10.8% respectively, p <0.005). Also, higher percentage of CD64+ CD80+ cells were identified over the total number of CD14+ cells in AAP over HPL (17.5% and 38.92% respectively, p <0.05), whereas no significant elevation of the percentage of CD163+ CD206+ cells was observed over the total number of CD14+ cells.
Conclusions: The results support an increase in the population of CD14+ cells and M1 over M2 macrophages in AAP versus HPL. This suggests a pro inflammatory profile and the progression of the AAP lesion.