IADR Abstract Archives
Effect of insulin on viability of fibroblasts on the titanium dental implants
Objectives: To analyze the effect of insulin on the proliferation and viability of fibroblasts on different surfaces of titanium dental implants into a cell culture medium.
Methods: Tree-Oss®, Biomet 3i® and Straumann® implant systems with different surface treatments were selected. The insulin concentration to cell proliferation was determined. Implants were incubated with normal cell line fibroblast (NIH-3T3) during 48 hours and 96 hours in two culture media: control (CTO, C3i and CST) and experimental (enriched with insulin; ITO, I3i and IST). After incubation, dental implants were fixed and stained with Hoestch techniques and a eosin stain. Implant surfaces were observed and photographed with an epifluorescence microscope. Cell viability (area percentage and integrity density) was estimated by ImageJ analysis software. The alkaline phosphatase metabolic activity (APA) was determined according to the Messer technique in the supernatants of cell cultures. The Kruskal Wallis test was used to compare control and experimental groups; Spearman coefficient was used to correlate cell proliferation with implant surface and ANOVA analysis was applied to determine differences between the variables. The Infostat software was used.
Results: The optimal insulin concentration in the two times was 20 nM. The mean values of area percentage of I3i group was higher than C3i group at 48 h (p<0.05) whereas no statistically significant values were observed in cell viability, neither between groups CTO/ITO, nor groups CST/IST. Statistically significant differences were found when comparing the area percentage of fibroblast between CTO and ITO (p=0.02) at 96 h, with higher values in the last one. It also was observed that integrity density values of IST and C3i groups were higher than CST and I3i groups, respectively, at 96 h of incubation (p<0.05). The mean values of APA of CTO and I3i groups were higher than ITO and C3i groups, respectively, at 48 h (p<0.05). Statistically significant values were observed at 96 h in the APA of IST group with respect to CST group (p<0.05).
Conclusions: The addition of insulin, the culture time and the surface characteristics influence the proliferation and viability of the fibroblasts in vitro on surfaces of dental implants.
Methods: Tree-Oss®, Biomet 3i® and Straumann® implant systems with different surface treatments were selected. The insulin concentration to cell proliferation was determined. Implants were incubated with normal cell line fibroblast (NIH-3T3) during 48 hours and 96 hours in two culture media: control (CTO, C3i and CST) and experimental (enriched with insulin; ITO, I3i and IST). After incubation, dental implants were fixed and stained with Hoestch techniques and a eosin stain. Implant surfaces were observed and photographed with an epifluorescence microscope. Cell viability (area percentage and integrity density) was estimated by ImageJ analysis software. The alkaline phosphatase metabolic activity (APA) was determined according to the Messer technique in the supernatants of cell cultures. The Kruskal Wallis test was used to compare control and experimental groups; Spearman coefficient was used to correlate cell proliferation with implant surface and ANOVA analysis was applied to determine differences between the variables. The Infostat software was used.
Results: The optimal insulin concentration in the two times was 20 nM. The mean values of area percentage of I3i group was higher than C3i group at 48 h (p<0.05) whereas no statistically significant values were observed in cell viability, neither between groups CTO/ITO, nor groups CST/IST. Statistically significant differences were found when comparing the area percentage of fibroblast between CTO and ITO (p=0.02) at 96 h, with higher values in the last one. It also was observed that integrity density values of IST and C3i groups were higher than CST and I3i groups, respectively, at 96 h of incubation (p<0.05). The mean values of APA of CTO and I3i groups were higher than ITO and C3i groups, respectively, at 48 h (p<0.05). Statistically significant values were observed at 96 h in the APA of IST group with respect to CST group (p<0.05).
Conclusions: The addition of insulin, the culture time and the surface characteristics influence the proliferation and viability of the fibroblasts in vitro on surfaces of dental implants.