MMP-13 and Elastase Expression in Human Gingiva with DM

Objectives: The purpose of this study is to exam the difference of pathologic condition by comparing expression phase of MMP-13, elastase and to find out the relationship of two proteins in gingival tissue of chronic periodontitis patient and those with type 2 diabetes mellitus. Methods: Study population consisted of 8 patients with type 2 diabetes and chronic periodontitis, 8 patients with chronic periodontitis, and 8 healthy individuals. Marginal gingival tissue samples were obtained by internal bevel incision at the time of periodontal surgery and prepared for western blotting. After western blotting, quantification analysis of MMP-13, elastase expression was performed using a densitometer and protein levels among each 3 groups were compared using one way ANOVA. Results: There was some tendencies that mean amount of MMP-13 was more increased in group 3 compared to group 1,2. Mean amount of elastase among three groups were similar. And two proteins didn't show statistical significance among three groups. Conclusions: MMP-13 expression was increased in inflamed gingiva with type 2 DM, therefore MMP-13 plays role in part in increased inflammation tendency of patient with type 2 DM. Elastase showed similar results between normal tissue and inflamed tissue. Further study in needed to confirm exact expression pattern of elastase in periodontitis.