Methods:Human pulp cell primary culture, ALP acitivity measurement, DSP Western Blot analysis
Results:1. The activity in the cells treated with PDGF AA showed lower levels than that in the control cells on days 5, 10, and 15, but the variations of activity throughout the culture period of PDGF AA treated cells were similar to those of control cells. In contrast, the ALP activity in the cells treated with PDGF AB or BB was significantly lower than that in the control group on days 5, 10, 15; the activity of each of these groups increased gradually, starting after day 10. 2. In DSP Western blotting, the PDGF AA treatment group showed decreased levels of these proteins compared with the control group. The expression in the PDGF AB treatment group was similar to that in the PDGF BB group, and the expression in both of these groups was significantly higher than that in the control group.
Conclusion:PDGF AA selectively inhibited the differentiation in the proliferative phase and had no effect on the latter differentiation process subsequent to the early process in the dental pulp cell culture, but PDGF AB and BB had effect on the latter differentiation process by inhibiting DSP expression.