Objectives:Ipriflavone (isoprofoxyisoflavone), a synthetic derivative from soy isoflavone daidzein has been shown to interfere with bone remodeling by inhibiting bone resorption and possibly stimulating bone formation. The present study aimed to elucidate effects of ipriflavone on the expression of extracellular matrix in rat calvarial cells in vitro.
Methods:The rat calvarial cells were isolated from fetus aged 20 to 21 days and cultured in BGJb media. 10-5 M, 10-6 M, 10-7 M, 10-8 M and 10-9 M concentration of ipriflavone was administered into each cultured cells. The cell proliferation was estimated through the measurement of MTT assay and the cellular activity was evaluated through the formation of the mineralization stained by Alizarin red S, and for detecting the expression of bone sialoprotein (BSP), type I collagen (COL I) and osteocalcin (OCN), RT-PCR analyses were done at 7 and 14 days after culture.
Results:1. There was no difference in the MTT assay of rat calvarial cell at any ipriflavone concentration.
2. The formation of mineralization was increased in concentration with 10-6 M, 10-7 M and 10-8 M ipriflavone.
3. The expression of BSP was incresed in concentration of ipriflavone from 7 day after culture.
4. The expression of COL I was incresed in concentration 10-6 M, 10-7 M and 10-8 M of ipriflavone from 7 days after culture.
5. The expression of OCN was markedly incresed in concentration of 10-6 M, 10-7 M and 10-8 M ipriflavone from 14 days after culture.
Conclusions:The present study indicates that ipriflavone might facilitate the rat calvarial cell differentiation and stimulate formation of mineralization. Thus, it suggests that ipriflavone may stimulate the bone remodeling process in early period of time.