IADR Abstract Archives
Curcumin and Bisdimethyl Curcumin Inhibit the Growth of A. actinomycetemcomitans
"Objective:" A. actinomycetemcomitans and antibiotic-resistant bacterial infection is the one of important problems in dental and medical fields. Therefore, new agents are needed to treat A. actinomycetemcomitans (A. actinomycetemcomitans) and methicillin-resistant Staphylococcus aureus (MRSA) from oral infection. In the present study, we investigated the antimicrobial activity of Curcuma longa (C. longa) and the antibacterial components, curcumin and bisdimethyl curcumin, from C. longa has been isolated and identified by MS, 1H-NMR, and 13C-NMR. "Methods:" C. longa was extracted with 80% MeOH, the extract was concentrated in vacua and the residue partitioned between H2O and CHCl3, EtOAc, and n-BuOH, respectively. The fractions of CHCl3 were assayed for growth inhibitory activity against A. actinomycetemcomitans ATCC strains 43717, 43718 and 33384, and MRSA. Column chromatography of the most active fraction on silica gel, Sephadex LH20, and prep-HPLC gave purified compounds. The structure of compounds was identified by MS, 1H-NMR, and 13C-NMR. "Results:" The CHCl3 fraction from solvent partition possessed greater antibacterial potential than other fractions. The fraction was separated by prep-HPLC to purified compounds. The compounds were identified as bisdimethyl curcumim and curcumim by MS, 1H-NMR, and 13C-NMR. The MICs of the fraction obtained from bisdimethyl curcumim and curcumim by column chromatography with silica gel and Sephadex LH20 to the A. actinomycetemcomitans ATCC strains 43717, 43718 and 33384 ranged from 0.25 to 0.125 mg/ml. The MICs of bisdimethyl curcumim and curcumim against MRSA were 0.125 mg/ml. "Conclusion:" These results suggest that curcumin and bisdimethyl curcumim isolated from C. longa by bioactivity-guided fractionation may have antibacterial activity against A. actinomycetemcomitans ATCC strains 43717, 43718 and 33384, and MRSA. This paper was supported by a grant No. R01-2001-000-00133-0 from Korean Science & Engineering Foundation.