Methods: The frozen sections of tongue, eye, salivary gland, and brain of ICR mouse were exposed to the hamster anti-mouse podoplanin and rat anti-mouse glial fibrillary acidic protein (GFAP), and to Alexa488/563-conjugated second antibodies. The sections were examined by the laser-scanning confocal microscopy.
Results: In the mouse tongue tissue, the strong reaction to the anti-podoplanin was observed on the perineurium around the nerve in the submucous layer and muscle layer, and on the myoepithelial cells of lingual glands. The strong reaction to the anti-podoplanin was also observed on the spinal ganglion and spinal nerve circumference at the cervical vertebrae. In the mouse eye, the reaction to anti-podoplanin was observed on the pigment epithelium, lens epithelium, corneal epithelium, conjunctival epithelium, and iris. In the brain, the reaction to anti-podoplanin was observed on the choroid plexus epithelium and some GFAP-positive astroglia.
Conclusions: Podoplanin is a common antigen on myoepithelium of salivary glands, oral mucous epithelium, perineurial fibroblasts, epithelial cells of eye, choroid plexus of brain, and some astroglia in the head and neck.