Influences of LPS upon the immunological response against metal ion

Objectives: Metallic allergies were epidemiologically correlated with periodontal diseases on human. However, the biochemical and the immunological aspects demonstrating correlation between periodontal diseases and metallic allergies have not elucidated, yet. In order to establish experimental chronic periodontitis, lipopolysaccharide (LPS), which is a gram (-) bacterial component affecting chronic periodontitis, was inoculated to mice. Then they were repeatedly sensitized with the metal materials, and influences affecting systemic immunities of these mice were analyzed.

Methods: C57BL/6J mice were inoculated with LPS in the 1st week. Then, these mice were sensitized with nickel chloride solution in the 3rd week, and then nickel sulfate solution was inoculated in the 6th week. In the 9th week, spleen cells from these mice were isolated. In order to stimulate T cells, spleen cells were cultured with anti-CD3ε monoclonal antibody for 48 hours; then the interferon (INF)-γ in the supernatants were determined by enzyme-linked immunosorbent assay (ELISA).

Results: The IFN-g productibity from spleen cells of the LPS-inoculated and nickel ion (Ni²⁺)-sensitized mice was augmented, while that of the Ni²⁺-sensitized. But not LPS-inoculated mice was reduced compared to that of untreated control mice. 

Conclusions: Our results have suggested that the chronic periodontitis, as a patient’s history, enhanced the immunological response against Ni²⁺ ion.Furthermore, we are analyzing the effects of components from several strains of Porphylomonas gingivalis upon metallic allergies.