Methods: Human fibroblasts (DS Pharma Biomedical Co., Ltd) were plated at 300 cells/well in 96well plates using plan DMEM supplemented with 10% fetal bovin serum.After 24 hours incubation with 5% CO2 at 37°C, cells were irradiated with non-thermal atmospheric micro plasma devices on the bottom of each 96well plates with energy dose of Low volt group(600volt, 30s, air flow10l/min: LV group) and High volt group(975volt, 30s, Air flow 10l/min: HV group).Control group was maintained at the micro plasma for the same irradiation times used in the respective irradiated groups, though without activating the micro plasma source.Seventy-two hours after the last irradiation, cells were evaluated metabolic activity, by means of MTT assay, cell number (trypan blue assay) and cell migration capacity (would healing assay). Additionally, fibroblast growth factor-2 (FGF-2) and Type±collagen prelease were measured in collected medium.Statistical analyses were performed using the excel statistical program file Ystat 2008(developed by Yamazaki S, Igakutosyo Syuppan co.ltd, Tokyo, Japan). Data were analyzed by the Mann-Whitney U-test with Bonferroni correction with statistical significance of 5%.
Results: The micro plasma irradiation groups(LV and HV group) increased a cell number, cell metabolism activity, collagen-producing quantity, FGF-2-producing quantity in significance in comparison with control group .
Conclusions: These findings of the present study demonstrated that the micro plasma irradiation increased metabolism activity.