Methods: THP-1 cells were cultured in RPMI 1640 medium supplemented with 10% fetal bovine serum. Cells were seeded at a density of 1.0×106cells/well , and incubated with methyl methacrylate (MMA), 2-hydroxyethyl methacrylate (HEMA), tetrathyleneglycol dimethacrylate (TEGDMA), or nickel (II) sulfate (NiSO4) at various concentrations for 24 h. Then, cells were stained with FITC-labeled CD54, CD86, and IgG antibodies for 30 min. The expression level of CD54 and CD86 were analyzed by flow cytometry. The statistical analyses of obtained date were performed using a two-tailed Student’s t-test.
Results: MMA showed no significant expression of CD54 at tested concentrations, whereas expression level of CD86 was increased over the concentration of 4000μg/mL accompanied with the reduction of cell viability. The expression level of both CD54 and CD86 were increased by the exposure of HEMA at the concentration of 500 μg/mL. TEGDMA showed significantly high expression level of CD54 at the concentration of 500 μg/mL, and the expression level of CD86 was increased over the concentration of 1200 μg/mL. NiSO4showed the highest expression level of both CD54 and CD86 at the lowest concentration (20 μg/mL), indicating its strong sensitization.
Conclusions: In this study, sensitizing potentiality of commonly utilized resin components (MMA, HEMA, and TEGDMA) were evaluated by h-CLAT, and it was revealed that these resin components showed weaker sensitizing potentiality than NiSO4.