Comparison of Biofilm on Different Subtrate Surface Topography
Objectives: The purpose of this study is to investigate the effects of substrate topography in vitro on biofilm formation of Streptoccocus mutans cultured with saliva on composite resin disc. Methods: Composite resin discs were formed with metal molds to achive uniform surface roughness and surface free energy. Four different types of discs were formed according to surface topography; flat surface, flat surface with 1-notch, 2-notches, 4-notches. The surface roughness of the discs was examined with confocal laser microsocopy and confirmed with scanning electronic microscopy. S. mutans was grown with each disc in biofilm media with either glucose or sucrose as energy source in the presence of fluid-phase or surface-adsorbed saliva for 24 hours. The adherent bacteria were quantified via enumeration of the total viable counts of bacteria. Results: There were significant differences in biofilm development according to surface topography. Compared to flat surface, biofilm formation was significantly increased with increasing numbers of notches. In addition, the differences in the amounts of biofilm formation were significantly influenced by the presence of fluid-phase or surface-adsorbed saliva, indicating saliva state in oral environment play different roles in prevention of biofilm formation. Conclusion: This study suggests that surface topography-notches on macroscopic scale may play an important role in biofilm formation of S. mutans.
Japanese Division Meeting
2011 Japanese Division Meeting (Hiroshima, Japan) Hiroshima, Japan
2011
Scientific Groups
Lee, Yoo Mee
( Seoul National University, Seoul, N/A, South Korea
)
Jun, Soo Kyung
( Seoul National University, Seoul, N/A, South Korea
)
Ahn, Joon Hyun
( Seoul National University, Seoul, N/A, South Korea
)
Nam, Shin Eun
( Seoul National University, Seoul, N/A, South Korea
)
Kim, Hong Kyun
( Seoul National University, Seoul, N/A, South Korea
)
Park, Young Seok
( Seoul National University, Seoul, N/A, South Korea
)
Lee, Seung Pyo
( Seoul National University, Seoul, N/A, South Korea
)