The plant hormone induces α-amylase expression in adipose-derived stem cells
Objectives: Salivary α-amylase is the most important enzyme for oral digestion of dietary starch. Therefore, regeneration of the salivary glands via a tissue engineering approach is clearly required for patients with salivary gland dysfunction. Early during seed germination, the embryo synthesizes a plant hormone (PH) that activates the synthesis and secretion of α-amylase. The purpose of this study was to explore the approach for differentiation of stem cells into salivary glands using PH.
Methods: We first isolated adipose-derived stem cells (ASCs), which have positive mesenchymal stem cell markers (CD73, CD90, and CD105) and pluripotency to osteoblasts, adipocytes, and neural cells, from human buccal fat pads, which are a readily available source for dentists and oral surgeons. We then investigated the cytotoxicity of PH for human ASCs. ASCs were incubated with PH to assess mRNA and protein expression of α-amylase by reverse transcriptase-polymerase chain reaction and Western blot analyses.
Results: PH neither affects cell morphology nor cell viability in a dose- or time-dependent manner. α-amylase mRNA expression on 21 days after treatment with PH (1 mM) was seven times greater than that in resting condition (0 day). While we did not detect α-amylase bands on 0 day, α-amylase protein was evident 7 days after treatment with PH, reaching a maximal level on day 21.
Conclusion: Our results indicated that PH can increase cellular α-amylase expression and that our induction method would be useful for therapeutic application for salivary gland regeneration.
Division: Japanese Division Meeting
Meeting:2011 Japanese Division Meeting (Hiroshima, Japan) Location: Hiroshima, Japan
Year: 2011 Final Presentation ID: Abstract Category|Abstract Category(s):Scientific Groups
Authors
Kasamatsu, Atsushi
( Graduate School of Medicine, Chiba University, Chiba, N/A, Japan
)
Uzawa, Katsuhiro
( Graduate School of Medicine, Chiba University, Chiba, N/A, Japan
)
Iyoda, Manabu
( Graduate School of Medicine, Chiba University, Chiba, N/A, Japan
)
Usukura, Katsuya
( Graduate School of Medicine, Chiba University, Chiba, N/A, Japan
)
Sakamoto, Yosuke
( Graduate School of Medicine, Chiba University, Chiba, N/A, Japan
)
Ogawara, Katsunori
( Graduate School of Medicine, Chiba University, Chiba, N/A, Japan
)
Shiiba, Masashi
( Graduate School of Medicine, Chiba University, Chiba, N/A, Japan
)
Tanzawa, Hideki
( Graduate School of Medicine, Chiba University, Chiba, N/A, Japan
)