The plant hormone induces α-amylase expression in adipose-derived stem cells
Objectives: Salivary α-amylase is the most important enzyme for oral digestion of dietary starch. Therefore, regeneration of the salivary glands via a tissue engineering approach is clearly required for patients with salivary gland dysfunction. Early during seed germination, the embryo synthesizes a plant hormone (PH) that activates the synthesis and secretion of α-amylase. The purpose of this study was to explore the approach for differentiation of stem cells into salivary glands using PH.
Methods: We first isolated adipose-derived stem cells (ASCs), which have positive mesenchymal stem cell markers (CD73, CD90, and CD105) and pluripotency to osteoblasts, adipocytes, and neural cells, from human buccal fat pads, which are a readily available source for dentists and oral surgeons. We then investigated the cytotoxicity of PH for human ASCs. ASCs were incubated with PH to assess mRNA and protein expression of α-amylase by reverse transcriptase-polymerase chain reaction and Western blot analyses.
Results: PH neither affects cell morphology nor cell viability in a dose- or time-dependent manner. α-amylase mRNA expression on 21 days after treatment with PH (1 mM) was seven times greater than that in resting condition (0 day). While we did not detect α-amylase bands on 0 day, α-amylase protein was evident 7 days after treatment with PH, reaching a maximal level on day 21.
Conclusion: Our results indicated that PH can increase cellular α-amylase expression and that our induction method would be useful for therapeutic application for salivary gland regeneration.
Japanese Division Meeting
2011 Japanese Division Meeting (Hiroshima, Japan) Hiroshima, Japan
2011
Scientific Groups
Kasamatsu, Atsushi
( Graduate School of Medicine, Chiba University, Chiba, N/A, Japan
)
Uzawa, Katsuhiro
( Graduate School of Medicine, Chiba University, Chiba, N/A, Japan
)
Iyoda, Manabu
( Graduate School of Medicine, Chiba University, Chiba, N/A, Japan
)
Usukura, Katsuya
( Graduate School of Medicine, Chiba University, Chiba, N/A, Japan
)
Sakamoto, Yosuke
( Graduate School of Medicine, Chiba University, Chiba, N/A, Japan
)
Ogawara, Katsunori
( Graduate School of Medicine, Chiba University, Chiba, N/A, Japan
)
Shiiba, Masashi
( Graduate School of Medicine, Chiba University, Chiba, N/A, Japan
)
Tanzawa, Hideki
( Graduate School of Medicine, Chiba University, Chiba, N/A, Japan
)
