IADR Abstract Archives

Effects of ultrasound and EDBL on cytotoxity in Ca9-22

Objectives: polyethylene glycosylated (PEG) liposome avoids uptake by the reticuloendothelial system, thus improving drug delivery to tumor while decreasing systemic toxicity. PEG liposomes containing doxorubicin, Doxil®, have been used to treat Kaposi's sarcoma and ovarian cancer in clinics. Doxil® circulates around the body at a stable state and accumulates in tumor by passive targeting. Although it is difficult to release doxorubicin after accumulation into the tumor, we made new bubble liposomes from Doxil® and investigated the effect on cytotoxicity in human gingival squamous cell carcinoma Ca9-22 cells by combination with this new Doxil® bubble liposome, encapsulated doxorubicin bubble liposomes (EDBL), and ultrasound.

Methods: EDBL were prepared with Doxil® and perfluoropropane gas. Intracellular deliveries of doxorubicin were performed by sonoporation with Doxil® or EDBL. Cell viability was determined by staining with trypan blue dye. The cell cycle was analyzed by a flow cytometer. Cells were cultured for 24 h and stained Hoechst 33342 to detect apoptotic cells.

Results: Cell viability was significantly reduced by combination of EDBL and ultrasound treatment. EDBL treated with ultrasound led to an augmentation of the proportion of the cells in the sub G1 phase. A DNA fragmentation was seen at 24 h after EDBL and ultrasound treatment.

Conclusion: The combination of EDBL and ultrasound was found to enhance the cytotoxity of Ca9-22 cells treated by doxorubicin. These findings suggest that combination of EDBL and ultrasound might be an effective drug delivery system against cancer cells.


Japanese Division Meeting
2010 Japanese Division Meeting (Kitakyushi City, Japan)
Kitakyushi City, Japan
2010

Scientific Groups
  • Iwanaga, Kenjiro  ( Kyushu Dental College, Kitakyushu, N/A, Japan )
  • Takahashi, Osamu  ( Kyushu Dental College, Kitakyushu, N/A, Japan )
  • Okinaga, Toshinori  ( Kyushu Dental College, Kitakyushu, N/A, Japan )
  • Kawano, Michinao  ( Kyushu Dental College, Kitakyushu, N/A, Japan )
  • Ariyoshi, Wataru  ( Kyushu Dental College, Kitakyushu, N/A, Japan )
  • Nishihara, Tatsuji  ( Kyushu Dental College, Kitakyushu, N/A, Japan )
  • Tominaga, Kazuhiro  ( Kyushu Dental College, Kitakyushu, N/A, Japan )
    • Pharmacology/Therapeutics/Toxicology