Methods: The myeloma cell line HS-72 was treated with HDACi (25-200 ng/ml) for 48 h. Cell viability was determined by WST-1 assay. The cell-cycle was analyzed by a flow cytometer. Western blot analysis was used to detect the expression of cell-cycle regulatory proteins, p21, Rb and CDK4, 6.
Results: HDACi inhibited the cell viability in dose-dependent manner in WST-1 assay. Flow cytometer analysis showed that HDACi induced a G1 phase cell-cycle arrest in HS-72. HDACi-treatment was found to upregulate the expression p21, downregulate CDK6, and induce phosphorylated Rb by Western blotting analysis.
Conclusion: Our results indicated that HDACi induced G1 cell cycle arrest through the regulation of protein related to cell cycle in myeloma cells.