P2Y₁₁ Purinoceptors Mediates the Chemotaxis Response in Rat Neutrophils

Objectives: The effect of purinoceptors signal on chemotaxis responses of rat neutrophils remains to be elucidated. Here, we have investigated whether these receptors are involved to increase the chemotaxis response. Methods: Rat neutrophils were prepared from male Wistar rats. The chemotaxis responses of rat neutrophils were stimulated by bacterial peptide, N -formyl-methionyl-leucyl-phenylalanine (fMLP) and measured with EZ-TAXIScan apparatus. Results: Exogenous application of ATP enhances the chemotaxis of cells stimulated by fMLP. The application of non-specific purinoceptor antagonist suramin and P2Y purinoceptors antagonist reactive blue 2, significantly inhibited the chemotaxis responses of rat neutrophils, respectively. The addition of apyrase, which hydrolyzes ATP, reduces the migration speed of rat neutrophils. These results suggest that activation of the fMLP stimulates localized ATP release, resulting in activation of purinoceptors and facilitates the chemotaxis responses. Pretreatment with selective antagonist of P2Y₁₁ purinoceptors NF157, significantly inhibited the chemotaxis responses. However, the application of P2X purinoceptors blocker PPADS and selective P2X₁ and P2X₃ purinoceptors antagonist TNP-ATP did not affect the chemotaxis responses. Blocked of A₃ adenosine receptor by MRS1191 did not affect the chemotaxis response. Conclusion: P2Y₁₁ purinoceptors play an importance of nucleotides in neutrophil chemotaxis response in rat.