Materials and Methods: A total of 24 cylindrical enamel specimens with 2-mm diameter were cut from the labial surface of bovine incisors, and these surfaces were polished with 600-1200 grit carbide papers. Half parts of the surfaces were then covered with nail varnish to maintain intact areas and demineralized in a 1% lactic acid solution for 48 hr to form erosive lesions. The specimens were then immersed for 24 hours in the human saliva samples allocated in 4 groups A-D as: human saliva only as control (A), saliva with 2% n-CAP (B), saliva with 1 ppm fluoride as NaF (C) and saliva with 1ppm fluoride plus 2% n-CAP. Human saliva samples were collected from 9 healthy adults, and were centrifuged at 3,000 r.p.m for 10 min before required. Finally, all the samples were assessed by the QLF to quantify mineral changes.
Results: The mean ΔF (±SD) values in each group were A: 34.8 ± 7.0%, B: 21.8 ± 5.7%, C: 30.9 ± 4.8%, and D: 34.5 ± 8.1%, and the group B showed significantly higher recovery in ΔF compared to the groups A and C (p<0.05; Tukey test).
Conclusions: In conclusion, it was suggested that n-CAP could remineralize erosive enamel lesions when applied by 2% with human saliva.