IADR Abstract Archives

Hyaluronic acid enhances BMP-2 induced osteogenesis in MG63 cells

Objectives: BMP-2 is expected to be utilized to fill bone defects and promote healing of fractures. However, it is unable to generate an adequate clinical response for use in bone regeneration. Recently, it was reported that glycosaminoglycan, including heparin, heparan sulfate, keratan sulfate, dermatan sulfate, chondroitin-4-sulfate, chondroitin-6-sulfate, and hyaluronic acid (HA), regulate BMP activity. However, the mechanism by which HA regulates osteogenic activities have not been fully elucidated. The aim of this study is to demonstrate the effect of HA on osteoblast differentiation induced by BMP-2.

Materials and Methods: Monolayer culture of osteoblastic lineage cell, MG63 cells were incubated with BMP-2 and HA for indicated time. To define the osteoblastic differentiation, ALP activity in cell lysate was quantified. Phosphorylation of Smad 1/5/8, p38, ERK proteins were determined by western blot analysis. To elucidate the nuclear translocation of phosphorylated Smad 1/5/8, stimulated cells were subjected to immnofluoresce microscopy. To further substantiate the role of HA on enhancement of BMP-2-induced Smad signaling, the mRNA expressions of BMP-2 receptor antagonist Noggin, and Follistatin were detected by real time RT-PCR.

Results: BMP-2-induced ALP activation, Smad 1/5/8 phosphorylation and nuclear translocation were increased when the cells were cultured with both BMP-2 and HA. Western blot analysis revealed that phosphorylation of ERK protein was diminished by HA. The mRNA expression of Noggin and Follistatin induced by BMP-2 were preferentially blocked by HA.

Conclusions: These results indicated that HA enhanced BMP-2 induced osteoblastic differentiation in MG63 cells, through the down-regulation of BMP-2 antagonist and ERK phosphorylation.


Japanese Division Meeting
2010 Japanese Division Meeting (Kitakyushi City, Japan)
Kitakyushi City, Japan
2010

Scientific Groups
  • Kawano, Michinao  ( Kyushu Dental College, Kitakyushu, N/A, Japan )
  • Ariyoshi, Wataru  ( Kyushu Dental College, Kitakyushu, N/A, Japan )
  • Iwanaga, Kenjiro  ( Kyushu Dental College, Kitakyushu, N/A, Japan )
  • Okinaga, Toshinori  ( Kyushu Dental College, Kitakyushu, N/A, Japan )
  • Habu, Manabu  ( Kyushu Dental College, Kitakyushu, N/A, Japan )
  • Tominaga, Kazuhiro  ( Kyushu Dental College, Kitakyushu, N/A, Japan )
  • Nishihara, Tatsuji  ( Kyushu Dental College, Kitakyushu, N/A, Japan )
    • Mineralized Tissue