Methods: Uterine squamous cell carcinoma was transplanted into the dorsal subcutaneous tissue of BALB/c nude mice. After transplantation, the animals were randomly divided into four equal groups. These groups received either no treatment (D-E-); 30mg/kg body weight bleomycin treatment without electroporation (D+E-); electroporation without bleomycin treatment (D-E+); or 30mg/kg body weight bleomycin treatment followed by electroporation (D+E+).
Results: These treatment performed four times of 0, 1, 7, 8 days, and the tumor volume were measured every other day for 14 days, and were investigated histologically. Electrical pulse treatment together with bleomycin injection markedly reduced the size of the tumor, whereas bleomycin injection or electrical pulse treatment alone did not.
Conclusions: These results clearly indicate that the antitumor effect of bleomycin on human uterine squamous cell carcinoma was considerably potentiated or enhanced by the administration of local electrical pulses at low voltages.