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Treponema denticola dentilisin involvement in periodontopathic bacterial coaggregation

Objectives: Treponema denticola, Tannerella forsythia and Porphyromonas gingivalis are frequently coisolated from periodontal lesions, and this combination of microorganisms has become known as the ‘red complex'. Coaggregation plays a key role in the organization of biofilm by these microorganisms. Although several adhesion factors have been identified for T. denticola, the ligand by which it achieves coaggregation remains to be determined. The purpose of this study was to clarify the component involved in coaggregation between T. denticola and T. forsythia.

Methods: T. forsythia ATCC43037 and T.denticola ATCC35405, ATCC33520, K1, KpSano and DMSP3 were used in this study. T.denticola K1 and KpSano are dentilisin-deficient mutants constructed by homologous recombination of wild-type strains T.denticola ATCC35405 and ATCC33520, respectively. T.denticola DMSP3 is a major outer sheath protein-deficient mutant constructed from T.denticola ATCC35405. Coaggregation between T. denticola and T. forsythia was evaluated by measuring OD660 using a spectrophotometer. The molecular mass of the ligand involved in coaggregation between T.denticola and T. forsythia was determined by overlay assay.

Results: Wild-type strains T.denticola ATCC35405 and ATCC33520 coaggregated with T. forsythia ATCC43037, whereas T.denticola K1 and KpSano decreased coaggregation reaction compared with wild-type strain about 55% and 45%, respectively. On the other hand, coaggregation was observed between T.denticola DMSP3 and T. forsythia ATCC43037.

Conclusion: The results suggest that dentilisin is involved in coagregation between T.denticola and T. forsythia.


Division: Japanese Division Meeting
Meeting: 2008 Japanese Division Meeting (Nagoya City, Japan)
Location: Nagoya City, Japan
Year: 2008
Final Presentation ID:
Abstract Category|Abstract Category(s): Scientific Groups
Authors
  • Sano, Yumiko  ( Tokyo Dental College, Chiba, N/A, Japan )
  • Miyamoto, Meguru  ( Tokyo Dental College, Chiba, N/A, Japan )
  • Ito, Rieko  ( Tokyo Dental College, Chiba, N/A, Japan )
  • Matsumoto, Naoya  ( Tokyo Dental College, Chiba, N/A, Japan )
  • Yakushiji, Masashi  ( Tokyo Dental College, Chiba, N/A, Japan )
  • Shintani, Seikou  ( Tokyo Dental College, Chiba, N/A, Japan )
  • Ishihara, Kazuyuki  ( Tokyo Dental College, Chiba, N/A, Japan )
    • SESSION INFORMATION
    Oral Session
    Microbiology / Immunology and Infection Control