Development and verification of a rapid oral bacteria detection system
Objectives: A rapid oral bacteria detection system was newly developed by Panasonic Shikoku Electronics Co., Ltd. for evaluation of oral hygiene by measuring the amount of oral bacteria. This device employed a dielectrophoretic impedance measurement (DEPIM) method that enabled measurement of the amount of oral bacteria easily and quickly (approximately within several tens of seconds), without using any reagents. The aim of this study was to examine the efficacy of this device for clinical application.
Methods: As an exploratory experiment, various concentrations of Escherichia coli K-12 strain as a standard specimen was measured using this device to confirm the measurement performance. After confirmation, we detected bacteria obtained from the dorsum of the tongue in 98 healthy adults and 251 dependent elderly persons, and evaluated the correlation between the measurements obtained by the DEPIM method and the existing culture method. In addition, we examined the effect of the pressure strength during sampling specimens on the number of bacteria obtained.
Results: The correlation coefficient between the bacterial concentrations calculated by the existing culture method and the DEPIM method in healthy adults and dependent elderly persons was R = 0.86 and R = 0.85, respectively, showing a high correlation. The number of oral bacteria in dependent elderly persons showed stronger correlation than that in healthy adults. In addition, there was a relationship between the pressure strength during sampling specimens and the number of oral bacteria. The number of oral bacteria increased with an increase in pressure strength.
Conclusions: It was suggested that the rapid oral bacteria detection system is effective for monitoring oral hygiene for clinical application.
This study was partly supported by a Grant-in-Aid from the Japanese Ministry of Health, Labor and Welfare (H19-choju-011) in 2007.
Division: Japanese Division Meeting
Meeting:2008 Japanese Division Meeting (Nagoya City, Japan) Location: Nagoya City, Japan
Year: 2008 Final Presentation ID: Abstract Category|Abstract Category(s):Scientific Groups
Authors
Kikutani, Takeshi
( Nippon Dental University Hospital, Tokyo, N/A, Japan
)
Kayanaka, Hisae
( Nippon Dental University, Tokyo, N/A, Japan
)
Nishiwaki, Keiko
( Nippon Dental University, Tokyo, N/A, Japan
)
Tamura, Fumiyo
( Nippon Dental University Hospital, Tokyo, N/A, Japan
)
Sekino, Satoshi
( Nippon Dental University, Tokyo, N/A, Japan
)
Hisano, Akiko
( Nippon Dental University Hospital, Tokyo, N/A, Japan
)
Konishi, Kiyoshi
( Nippon Dental University School of Life Dentisty at Tokyo, Tokyo, N/A, Japan
)
Yoshida, Mitsuyoshi
( Hiroshima University, Hiroshima, N/A, Japan
)