Effects of Obesity on Oxidative Stress in Rat Gingiva
Objectives: Studies have suggested a correlation between obesity and periodontal disease. It has been shown that oxidative stress is involved in progression of periodontitis. The purpose of this study was to investigate the effects of obesity on gingival oxidative stress in rat periodontitis model.
Methods: The obese Zucker rats (n= 14) and their lean littermates (n= 14) were divided into two groups of 7 rats, respectively. In one of each group, periodontitis was ligature-induced for 4 wks, while the other group was left unligated. In order to examine gingival oxidative stress, the level of 8-hydroxydeoxyguanosine (8-OHdG) and the ratio of reduced/oxidized glutathione were determined. Serum level of hydroperoxides was measured by a free radical electric evaluator. In addition, the gingival gene expression pattern related to oxidative/metabolic stress, inflammation and cell behavior was evaluated using real-time polymerase chain-reaction array technology.
Results: In comparison with the lean rats without periodontitis, the obese rats without periodontitis showed a 57% increase in 8-OHdG level (p< 0.05) and an 18% decrease in reduced/oxidized glutathione ratio (p< 0.05) in the gingival tissue, with increasing serum level of hydroperoxides (p< 0.05). The obese rats with periodontitis also increased 8-OHdG level by 61% (p< 0.05) and decreased reduced/oxidized glutathione ratio by 37% (p< 0.05) compared to the lean rats with periodontitis. Furthermore, in the periodontal lesions, the expression of multiple cytochrome P450 genes were more than 2-fold down-regulated by obesity.
Conclusions: Obesity induces gingival oxidative stress by increasing serum hydroperoxides. In addition, a reduced capacity for xenobiotic detoxification in the gingival tissue may be involved in augmentation of periodontitis-induced oxidative stress in the obese model.
Division: Japanese Division Meeting
Meeting:2008 Japanese Division Meeting (Nagoya City, Japan) Location: Nagoya City, Japan
Year: 2008 Final Presentation ID: Abstract Category|Abstract Category(s):Scientific Groups
Authors
Tomofuji, Takaaki
( Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, N/A, Japan
)
Kokeguchi, Susumu
( Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, N/A, Japan
)
Yamamoto, Tatsuo
( Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, N/A, Japan
)
Morita, Manabu
( Okayama University, Okayama, N/A, Japan
)
Sanbe, Toshihiro
( Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, N/A, Japan
)
Tamaki, Naofumi
( Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, N/A, Japan
)
Ekuni, Daisuke
( Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, N/A, Japan
)
Kasuyama, Kenta
( Okayama University, Okayama, N/A, Japan
)
Umakoshi, Michihiro
( Okayama University, Okayama, N/A, Japan
)
Irie, Koichiro
( Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, N/A, Japan
)
Azuma, Tetsuji
( Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, N/A, Japan
)
Murakami, Jun
( Okayama University, Okayama, N/A, Japan
)