Histomorphometric Analysis of rhBMP-2 on Edentulos Ridge in Rats
Objective: BMP is the most promising osteoinductive substance and is expected to be applied clinically for bone reconstruction.However, it has not been successfully achieved about reconstruction of edentulous ridge.This study was designed to evaluate the effect of BMP to edentulous ridge in rats.Methods: Upper bilateral first and second molars of 8week-old Wistar rats were extracted 3 weeks before implantation.Compressed polylactic acid polyglycolic acid copolymer/gelatin sponge (PGS) was used as carrier of BMP.PGS with/without BMP (5µg) was implanted onto the alveolar ridge from anterior incision of the ridge (BMP group, PGS group).Bone healing sites were used as controls.The rats were killed 1,2,4,8 and 12 weeks after implantation and examined histomorphometrically.Fluorescent labeled alveolar ridges were stained with Villanueva bone stain and were prepared for the undecalcified grinding sections.Differences of primary measurement, structural index and dynamic variables were analyzed by two-way ANOVA.Results: In the BMP group, Osteoid surface increased at 2 and 4 weeks, and Osteoid thickness became the maximum (25µm) on 2 weeks.Dynamic variables indicated calcification became the maximum on 4 weeks and then decreased.However, they were larger than PGS group and control group at 4 and 8 weeks (p<0.05).New bone was matured until 12 weeks.Consequently, from the 2 weeks on, Tissue volume and Height of alveolar bone were larger than PGS group and control group at 4 and 8 weeks (p<0.05).On the other hand, each parameter in PGS group and control group remained stable and there were no significant differences.Conclusion: BMP induced new bone formation for a long term and PGS was an effective carrier for BMP.Our results suggested that BMP would be useful for bone reconstruction in edentulous ridge.
Division: Japanese Division Meeting
Meeting:2004 Japanese Division Meeting (Tokyo, Japan) Location: Tokyo, Japan
Year: 2004 Final Presentation ID:24 Abstract Category|Abstract Category(s):Scientific Groups
Authors
Shimazu, Chie
( Okayama University, Okayama City, N/A, Japan
)
Hara, Tetsuya
( Okayama University, Okayama City, N/A, Japan
)
Kinuta, Yoshihiro
( Okayama University, Okayama City, N/A, Japan
)
Moriya, Katsunori
( Okayama University, Okayama City, N/A, Japan
)
Maruo, Yukinori
( Okayama University, Okayama City, N/A, Japan
)
Hanada, Shunshi
( Okayama University, Okayama City, N/A, Japan
)
Minagi, Shogo
( Okayama University, Okayama City, N/A, Japan
)