IADR Abstract Archives

Artepillin C regulates extracellular matrix gene expressions in human periodontal fibroblasts by DNA methylation

Abstract: Objectives:
Artepillin C is the major component in Brazilian green propolis. As propolis exhibits antibacterial, antifungal, and antiviral properties, it is often used for the prevention of periodontal diseases. However, the mechanism through which propolis affects the periodontal tissue is still unknown. To elucidate the effect of artepillin C on periodontal tissue, we analyzed the expression level and DNA methylation status in human periodontal ligament fibroblasts (HPDLFs) upon artepillin C treatment.
Methods:
The culture of HPDLFs was repeated alternating 3 days with artepillin C (25 μM/ml, WAKO) and 3 days without artepillin C in DMEM containing 10% FBS for 1 month. Untreated samples were used as controls. We then performed microarray (Agilent technology) and quantitative RT-PCR analyses. The expression profile of the genes analyzed through microarray was classified by functional annotation with keyword analysis in Database for Annotation, Visualization, and Integrated Discovery (DAVID). The selected relevant genes were further analyzed for their DNA methylation status in the promoter regions through methylation-specific PCR.
Results:
Overall, 370 (1.5-fold) upregulated genes and 377 (0.66-fold) downregulated genes were identified upon artepillin C treatment via microarray analysis. Among these, 18 genes belonging to the extracellular matrix were significantly correlated in the keyword analysis in DAVID. Within the extracellular matrix genes, HSPG2 and COL5A3 exhibited significantly altered gene expression patterns and DNA methylation levels.
Conclusions:
These results indicate that artepillin C is responsible for the altered methylation status in the promoter region, resulting in differences in the expression patterns of the extracellular matrix-related genes.
Japanese Division Meeting
2020 Japanese Division Meeting (Virtual)
Virtual, Japan
2020
40
Oral Medicine & Pathology Research
  • Takai, Rie  ( Advanced Research Promotion Center, Health Sciences University of Hokkaido, Ishikari-Tobetsu, Hokkaido, JAPAN )
  • Uehara, O.  ( Division of Disease Control and Molecular Epidemiology, School of Dentistry, Health Sciences University of Hokkaido, Ishikari-Tobetsu, Hokkaido, JAPAN )
  • Morikawa, T.  ( Division of Oral Medicine and Pathology, School of Dentistry, Health Sciences University of Hokkaido, Ishikari-Tobetsu, Hokkaido, JAPAN )
  • Yoshida, K.  ( Division of Oral Medicine and Pathology, School of Dentistry, Health Sciences University of Hokkaido, Ishikari-Tobetsu, Hokkaido, JAPAN )
  • Sato, J.  ( Division of Oral Medicine and Pathology, School of Dentistry, Health Sciences University of Hokkaido, Ishikari-Tobetsu, Hokkaido, JAPAN )
  • Abiko, Y.  ( Division of Oral Medicine and Pathology, School of Dentistry, Health Sciences University of Hokkaido, Ishikari-Tobetsu, Hokkaido, JAPAN )
  • Ohta, T.  ( Advanced Research Promotion Center, Health Sciences University of Hokkaido, Ishikari-Tobetsu, Hokkaido, JAPAN )
  • Poster Session