IADR Abstract Archives

Comparison of microbiome organized on denture base materials and hydroxyapatite

Abstract: Objectives: Oral microorganisms colonize oral surfaces by forming biofilms. Dentures sometimes act as reservoirs of bacteria in people with insufficient oral hygiene practices. Biofilms on the surface of dentures are thought to increase the risk of aspiration pneumonia in the elderly. However, how oral microbe communities organize into biofilms has yet to be fully clarified. In this study, we investigated the composition of biofilms on denture base materials in vivo.Methods: Thirteen healthy individuals were enrolled in this study. Disk shaped denture material specimens composed of polymethyl methacrylate (PMMA), cobalt-chromium (CoCr) alloy, and hydroxyapatite (HA) were worn by subjects for 48 h attached to an appliance in the oral cavity. The V3-V4 region of 16S rRNA coding sequences from bacteria in the biofilms that formed on the denture materials were sequenced by Mysec. Qiime2 and LEfSe analysis identified the operational taxonomical unit (OTU) in biofilms on each material and characterized the prevenance of the OTUs.Results: The average number of sequence reads per sample was 97,005 ± 91,523. Among the sequences from all samples, 328 OTUs were detected. The α diversity of the sample from CoCr was lower than that of HA; however, the difference was not significant. The composition of the microbiome did not differ at the genus level among the three materials, according to UniFrac analysis. Using LEfSe analysis, 10 genera showed differences between PMMA and HA.Conclusions: This study showed that each denture material supports the formation of a similar microbiome. This result suggests that the choice of material has little effect on the composition of the bacterial flora.
Japanese Division Meeting
2020 Japanese Division Meeting (Virtual)
Virtual, Japan
2020
19
Microbiology/Immunology
  • Nezu, Yuichi  ( Removable Prosthodontics & Gerodontology, Tokyo Dental College, Tokyo, Japan )
  • Ryu, M.  ( Removable Prosthodontics & Gerodontology, Tokyo Dental College, Tokyo, Japan )
  • Ishihara, K.  ( Microbiology, Tokyo Dental College, Tokyo, JAPAN )
  • Ueda, T.  ( Removable Prosthodontics & Gerodontology, Tokyo Dental College, Tokyo, Japan )
  • Poster Session