IADR Abstract Archives
Analysis of PLAP-1 in a mouse periodontitis model
Objectives: Periodontal ligament-associated protein 1 (PLAP-1)/asporin is an extracellular matrix protein preferentially expressed in periodontal ligaments. PLAP-1 plays important roles in the maintenance of periodontal tissue homeostasis. We previously reported that PLAP-1 has a defensive role in periodontitis lesions by suppressing pathophysiologic TLR signaling. However, the involvement of PLAP-1 in the progression of periodontal disease remains unknown. In this study, we analyzed the role of PLAP-1 in the progress of periodontal disease in PLAP-1 knockout (KO) mice and the experimental periodontitis model.
Methods: Mandibular bone was collected from 8 week-old male C57BL / 6 (WT) mouse and PLAP-1 KO mouse, respectively, and analyzed by micro computed tomography (micro CT) scanning ,hematoxylin and eosin (HE) staining and picrosirius red staining. Further, experimental periodontitis was induced by ligation with 8-0 silk threads to the second molar tooth of the maxillary left side for 14 days. Alveolar bone loss was evaluated by micro CT scanning. Histologic changes were detected by HE staining.
Results: In PLAP-1 KO mice, there was no significant difference in appearance compared to WT mice, but the volume of the periodontal ligament was significantly increased, and the collagen fiber density in the periodontal ligament was low. The PLAP-1 KO mouse in which experimental periodontitis was induced showed significant alveolar bone absorption compared to the WT mouse. In addition, fractured fibrin and blood vessel dilation were observed in the periodontitis lesion of the PLAP-1 KO mouse.
Conclusions: In the experimental periodontitis mouse model, it is suggested that PLAP-1 may play a protective role in progress of periodontitis by regulating physiological structure of periodontal ligament.
Methods: Mandibular bone was collected from 8 week-old male C57BL / 6 (WT) mouse and PLAP-1 KO mouse, respectively, and analyzed by micro computed tomography (micro CT) scanning ,hematoxylin and eosin (HE) staining and picrosirius red staining. Further, experimental periodontitis was induced by ligation with 8-0 silk threads to the second molar tooth of the maxillary left side for 14 days. Alveolar bone loss was evaluated by micro CT scanning. Histologic changes were detected by HE staining.
Results: In PLAP-1 KO mice, there was no significant difference in appearance compared to WT mice, but the volume of the periodontal ligament was significantly increased, and the collagen fiber density in the periodontal ligament was low. The PLAP-1 KO mouse in which experimental periodontitis was induced showed significant alveolar bone absorption compared to the WT mouse. In addition, fractured fibrin and blood vessel dilation were observed in the periodontitis lesion of the PLAP-1 KO mouse.
Conclusions: In the experimental periodontitis mouse model, it is suggested that PLAP-1 may play a protective role in progress of periodontitis by regulating physiological structure of periodontal ligament.