IADR Abstract Archives
Anti-gravity mechano-dynamic culture revealed an on-line 3D formation of bone matrix
Objectives: We have previously developed various three-dimensional (3D) scaffolds for osteoblast culture and the bone reconstruction technology including a honeycomb-shaped tricalcium phosphate and a titanium web. However, we noticed that the larger the volume of 3D scaffold, the less efficient was the penetration of nutrients in the scaffold. Therefore, we decided to develop a new device to achieve efficient circulation, and at the same time to provide some of the mechanical stimuli for cultured cells. Our hypothesis is as follows: if we change the direction of gravitational force on the cells systematically, we can provide the cells mechano-dynamic effects and better circulation of the medium. We aimed to prove the hypothsis.
Methods: We constructed a new device to invert the position of conventional culture dishes upside-down in the culture medium. This device can also rotate the 3D scaffold in a regular manner.
Results: The result showed that the amount of collagen production increased 4 times higher than the static contr
ol. In the present study, we culture the stem cells called DFAT cells (de-differentiated fat cells) upon the anti-gravity culture system. One of the remarkable finding was that not only the fibrous networks collagen, but also osteoid-like structures surrounded by osteoblasts were clearly observed.
Conclusions: These results indicates that combination of geometrical factor of 3D matrix, mechano-dynamic stimuli and better circulation of medium provided the stem cells a similar environment to in vivo condition.
Methods: We constructed a new device to invert the position of conventional culture dishes upside-down in the culture medium. This device can also rotate the 3D scaffold in a regular manner.
Results: The result showed that the amount of collagen production increased 4 times higher than the static contr
ol. In the present study, we culture the stem cells called DFAT cells (de-differentiated fat cells) upon the anti-gravity culture system. One of the remarkable finding was that not only the fibrous networks collagen, but also osteoid-like structures surrounded by osteoblasts were clearly observed.
Conclusions: These results indicates that combination of geometrical factor of 3D matrix, mechano-dynamic stimuli and better circulation of medium provided the stem cells a similar environment to in vivo condition.