IADR Abstract Archives
A Microbiological Study on Bacteria in the PET Bottles After Drinking
Objectives: It is suspected that oral bacteria grow in the PET bottles after drinking straight from bottles. In order to know the bacterial influence on the general health (e.g., food poisoning), as a first step in the present study, bacteria at the mouth of PET bottles after drinking were analyzed quantitively and qualitatively.
Methods: After obtaining informed consent, 5 healthy subjects (20-23 years) were asked to drink non-sugar tea or sports drinks (ca. 50 mL) in a PET bottle (of 300 mL). Both immediately after and one-day after drinking, the mouth of PET bottles was wiped with sterile cotton swabs. After suspended and vortexed in a buffer, serial-diluted samples were inoculated onto the CDC Anaerobe blood agar plates, and incubated anaerobically at 37°C for 7 days. Samples of one-day after drinking were also cultured. Genomic DNA were extracted from individual colonies, and bacterial species were identified by 16S rRNA gene sequencing.
Results: The mean amounts of bacteria (CFU/mL) were (2.2 ± 0.6)×104 and (9.4 ± 8.3)×103 from the mouth of plastic bottles (of the non-sugar tea) immediately after and one-day after drinking, respectively. On the other hand, (2.6 ± 1.5 )×106 were recovered from drinks of one-day after drinking. Streptococcus (42.8%) were predominant in the mouth of plastic bottles, followed by Actinomyces (13.0%), Propionibacterium (5.8%), Gemella (5.1%), Staphylococcus (5.1%) and Veillonella (5.1%). In contrast, Streptococcus were overwhelmingly detected one-day after drinking both in the non-sugar tea and sports drinks.
Conclusions: These results suggest that the composition in the mouth of plastic bottles was similar to that of the saliva reported previously (Ishida et al 2015), and that the predominant species survived in a drink one-day after drinking.
Methods: After obtaining informed consent, 5 healthy subjects (20-23 years) were asked to drink non-sugar tea or sports drinks (ca. 50 mL) in a PET bottle (of 300 mL). Both immediately after and one-day after drinking, the mouth of PET bottles was wiped with sterile cotton swabs. After suspended and vortexed in a buffer, serial-diluted samples were inoculated onto the CDC Anaerobe blood agar plates, and incubated anaerobically at 37°C for 7 days. Samples of one-day after drinking were also cultured. Genomic DNA were extracted from individual colonies, and bacterial species were identified by 16S rRNA gene sequencing.
Results: The mean amounts of bacteria (CFU/mL) were (2.2 ± 0.6)×104 and (9.4 ± 8.3)×103 from the mouth of plastic bottles (of the non-sugar tea) immediately after and one-day after drinking, respectively. On the other hand, (2.6 ± 1.5 )×106 were recovered from drinks of one-day after drinking. Streptococcus (42.8%) were predominant in the mouth of plastic bottles, followed by Actinomyces (13.0%), Propionibacterium (5.8%), Gemella (5.1%), Staphylococcus (5.1%) and Veillonella (5.1%). In contrast, Streptococcus were overwhelmingly detected one-day after drinking both in the non-sugar tea and sports drinks.
Conclusions: These results suggest that the composition in the mouth of plastic bottles was similar to that of the saliva reported previously (Ishida et al 2015), and that the predominant species survived in a drink one-day after drinking.