IADR Abstract Archives
Antibacterial and Protein-repellent Surface Coating Composed of MDPB and MPC
Objectives: 12-methacryloyloxydodecylpyrimidinium bromide (MDPB) is an antibacterial monomer, which was developed to be immobilized on the resinous restorative materials to achieve long-lasting antibacterial effects. However, the effectiveness of immobilized MDPB is reduced by coverage with salivary protein. To overcome such problem, we focused on utilization of 2-methacryloyloxyethyl phosphorylcholine (MPC) polymer which shows protein-repellent property. In this study, dual-functional surface coating composed of MDPB and MPC was fabricated, and its protein-repellent capability and antimicrobial effects were evaluated.
Methods: With 2,2’-azobisisobutyronitrile as an initiator, mixtures of MDPB, MPC, and butyl methacrylate (BMA) at 5 different weight ratios (MDPB/MPC/BMA = 0/30/70, 5/25/70, 15/15/70, 25/5/70, 30/0/70) were polymerized. Each copolymer was dissolved in ethanol and applied to a cured poly(methyl methacrylate) (PMMA) disc. To examine the ability to coat PMMA, FITC-labeled copolymers were used and the coated surfaces were observed by using a confocal laser scanning microscopy (CLSM). To evaluate protein-repellent capability, the adsorption of bovine serum albumin on the coated surface was analyzed by micro BCA protein assay and CLSM observation. For antibacterial tests, Streptococcus mutans suspension at 1 × 103 CFU/mL was placed on the coated specimen, and the bacterial number was counted after 24 h incubation.
Results: All copolymers prepared were found to be able to coat PMMA surfaces effectively. Copolymers containing MPC at ≥15% significantly reduced the adsorption of protein (p<0.05, ANOVA, Tukey’s HSD test). Copolymers with ≥5% of MDPB significantly inhibited the growth of S. mutans on their surfaces (p<0.05, ANOVA, Tukey’s HSD test), and those with ≥15% of MDPB exhibited complete killing effect.
Conclusions: The copolymer of 15(wt)% MDPB/15(wt)% MPC/70(wt)% BMA was effective to exhibit both protein-repellent capability and bactericidal effects against S. mutans. Thus, the novel dual-functional coating polymer composed of MDPB/MPC system is promising to help hinder biofilm formation on restorative materials.
Methods: With 2,2’-azobisisobutyronitrile as an initiator, mixtures of MDPB, MPC, and butyl methacrylate (BMA) at 5 different weight ratios (MDPB/MPC/BMA = 0/30/70, 5/25/70, 15/15/70, 25/5/70, 30/0/70) were polymerized. Each copolymer was dissolved in ethanol and applied to a cured poly(methyl methacrylate) (PMMA) disc. To examine the ability to coat PMMA, FITC-labeled copolymers were used and the coated surfaces were observed by using a confocal laser scanning microscopy (CLSM). To evaluate protein-repellent capability, the adsorption of bovine serum albumin on the coated surface was analyzed by micro BCA protein assay and CLSM observation. For antibacterial tests, Streptococcus mutans suspension at 1 × 103 CFU/mL was placed on the coated specimen, and the bacterial number was counted after 24 h incubation.
Results: All copolymers prepared were found to be able to coat PMMA surfaces effectively. Copolymers containing MPC at ≥15% significantly reduced the adsorption of protein (p<0.05, ANOVA, Tukey’s HSD test). Copolymers with ≥5% of MDPB significantly inhibited the growth of S. mutans on their surfaces (p<0.05, ANOVA, Tukey’s HSD test), and those with ≥15% of MDPB exhibited complete killing effect.
Conclusions: The copolymer of 15(wt)% MDPB/15(wt)% MPC/70(wt)% BMA was effective to exhibit both protein-repellent capability and bactericidal effects against S. mutans. Thus, the novel dual-functional coating polymer composed of MDPB/MPC system is promising to help hinder biofilm formation on restorative materials.