Eubacteria saphenum and TM7 in periodontal disease.

Objectives: Periodontal disease is the inflammatory condition of periodontal structure. The common disease factor is microorganism and metagenome approaches revealed the oral microbiota of periodontitis. Although many oral pathogens were identified in microbiome analysis, it was difficult to diagnose for periodontal disease. Recently, genomic analysis revealed gene information and it is able to measure uncultured bacteria using synthesis artificial gene by quantitative real-time PCR. Aim of this study was finding the new periodontitis pathogens for marker of periodontitis.
Methods: The stimulating whole saliva were collected from 172 periodontitis participants. Written informed consent was obtained from the patient before sampling. According to other microbiome analysis, Eubacterium saphenum, TM7, TM7taxon356 are selected for candidate pathogens. These bacteria were measured by the quantitative real-time PCR using the specific primer with Taqman probe. Major periodontopathic bacteria such as Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis, Prevotella intermedia, Treponema denticola, Tannerella forsythia and Fusobacterium nucleatum were also measured.
Results: Eubacterium saphenum was significantly higher in chronic severe periodontitis than healing group (P<0.001). The proportion of obligate anaerobe as Porphyromonas gingivalis, Treponema denticola, Tannerella forsythia and Prevotella intermedia, were significantly higher in chronic severe periodontitis group than chronic mild periodontitis and healing groups (P<0.001). Aggregatibacter actinomycetemcomitans, Fusobacterium nucleatum, TM7 and TM7 taxon356 were not significantly different with other groups.
Conclusions: The saliva samples were found the putative periodontal pathogens proportion of the “red complex” and “orange complex” that were reported in the past as Porphyromonas gingivalis, Treponema denticola Tannerella forsythia and Prevotella intermedia were significantly higher in chronic severe periodontitis. Eubacterium saphenum was observed in almost severe periodontitis patients and associated with proportion of Porphyromonas gingivalis. In addition to red complex of periodontal pathogens, Eubacterium saphenum might be the new marker for identified predominantly chronic periodontal disease.