IADR Abstract Archives

Hyaluronic Acid/Peptide-Based Hydrogel Induces Bone Regeneration in Calvarial Critical-Sized Defects

Objectives: Biomimetic materials that can stimulate and accelerate bone formation in vivo to treat critical-sized bone defects are of great interest. Herein, the ability of an extracellular matrix-mimicking hydrogel of calvarial bone regeneration is demonstrated.
Methods: Hyaluronic acid (HA) was integrated with an ultra-short self-assembling aromatic peptide hydrogelator to form a biomimetic fibrous hydrogel. The hydrogel was placed in a 5-mm calvarial bone defect compared to a non-filled defect (negative control) and a defect filled with deproteinized bovine bone mineral (BBM) (positive control). Eight weeks post-surgery, the calvarias were harvested and the restored bone volume, density, and thickness were calculated relative to the contralateral intact side of the calvaria using Micro Computed Tomography.
Results: In the HA/peptide hydrogel group, the restored bone volume was 92.95 ± 29.30%. Statistically significant lower values of 37.89 ± 19.02% (p = 0.0256) and 41.20 ± 27.24% (p = 0.0355) were observed in the unfilled defect and the BBM groups, respectively. Coronal cross-sections of the defects in the three groups revealed that unfilled defects contained newly formed bone only at the defect margins, while, in the defects filled with HA/peptide or BBM, the newly formed bone bridged the defect. Notably, only in the HA/peptide-filled defect, the newly formed mineralized tissue was indistinguishable from the surrounding native bone. The thickness of the newly formed bone was homogeneous throughout the defect with a similar or even higher thickness than on the untreated control side.
Conclusions: The hydrogel served as a temporary self-supporting scaffold and resulted in a complete restoration of the calvaria's original thickness. Interestingly, the scaffold not only induced bone deposition around the margins but also generated bony islets in the central part of the defect.

2021 Israeli Division Meeting (Jerusalem, Israel)

2021

  • Halperin-sternfeld, Michal  ( Tel Aviv University Sackler Faculty of Medicine , Tel Aviv , Israel )
  • Adler-abramovich, Lihi  ( Tel Aviv University Sackler Faculty of Medicine , Tel Aviv , Israel )
  • Pokhojaev, Ariel  ( Tel Aviv University Sackler Faculty of Medicine , Tel Aviv , Israel )
  • Ghosh, Moumita  ( Tel Aviv University Sackler Faculty of Medicine , Tel Aviv , Israel )
  • Rachmiel, Dana  ( Tel Aviv University Sackler Faculty of Medicine , Tel Aviv , Israel )
  • Kannan, Raha  ( University of Michigan , Ann Arbor , Michigan , United States )
  • Grinberg, Itzhak  ( Tel Aviv University Sackler Faculty of Medicine , Tel Aviv , Israel )
  • Ma, Peter  ( University of Michigan , Ann Arbor , Michigan , United States )
  • Binderman, Itzhak  ( Tel Aviv University Sackler Faculty of Medicine , Tel Aviv , Israel )
  • Sarig, Rachel  ( Tel Aviv University Sackler Faculty of Medicine , Tel Aviv , Israel )
  • None
    Clore Scholarship, BSF (grant No. 2018102) , ISRAEL SCIENCE FOUNDATION (grant No. 1732/17), European Research Council (ERC) under the European Union’s Horizon 2020 research and innovation programme (grant agreement No. 948102).
    Oral Session
    Oral Session 1