IADR Abstract Archives
Analysis of Oral Microbiome in Bariatric Surgery Patients
Objectives: obesity and bariatric surgery can affect the oral health status, and post-surgery alterations can reflect in the oral cavity. However, the essence and nature of these alterations in oral conditions is still not conclusive. Here we aim to compare the oral microbiota and oral health status of bariatric surgery patients pre- and post-operation divided into four “oral” groups: gingivitis, caries, periodontitis, and periodontitis+ caries compared to systemically healthy volunteers.
Methods: The oral status of pre-bariatric surgery patients and systemically healthy volunteers was examined and categorized accordingly to one of the two “oral” groups. Next, by employing next-generation sequencing of 16S rDNA, the oral microbiome was explored and assessed for a specific set of unique microorganisms to each population. Follow-up one year after surgery, the oral health status and microbiome content of bariatric surgery patients was reexamined.
Results: 56 systematically healthy volunteers, 35 bariatric–pre-surgery patients, and 15 bariatric– 1-year post-surgery patients were examined. Alpha diversity was relatively higher in bariatric patients than systemically healthy volunteers and in the periodontitis + caries group compared to the other “oral” groups. In the beta diversity test, microbial composition differed between the four “oral” groups as well as between bariatric patients and systemically healthy volunteers. Relative to systemically healthy volunteers, the B/P ratio (Bacteroidota/Proteobacteria) was higher in bariatric patients, with significant shifts in these six bacterial taxa: Pseudomonas, Streptococcus, Hemophilus, Neisseria, Prevotella, Serratia.
Conclusions: The higher B/P ratio in the bariatric group is indicative of microbiome dysbiosis. In addition, periodontitis + caries caused a more significant shift in microbial diversity. Moreover, microbiome analysis showed a distinct cluster of the bariatric group. Our results suggest that oral microbiome and oral diseases are affected by obesity and weight loss.
Methods: The oral status of pre-bariatric surgery patients and systemically healthy volunteers was examined and categorized accordingly to one of the two “oral” groups. Next, by employing next-generation sequencing of 16S rDNA, the oral microbiome was explored and assessed for a specific set of unique microorganisms to each population. Follow-up one year after surgery, the oral health status and microbiome content of bariatric surgery patients was reexamined.
Results: 56 systematically healthy volunteers, 35 bariatric–pre-surgery patients, and 15 bariatric– 1-year post-surgery patients were examined. Alpha diversity was relatively higher in bariatric patients than systemically healthy volunteers and in the periodontitis + caries group compared to the other “oral” groups. In the beta diversity test, microbial composition differed between the four “oral” groups as well as between bariatric patients and systemically healthy volunteers. Relative to systemically healthy volunteers, the B/P ratio (Bacteroidota/Proteobacteria) was higher in bariatric patients, with significant shifts in these six bacterial taxa: Pseudomonas, Streptococcus, Hemophilus, Neisseria, Prevotella, Serratia.
Conclusions: The higher B/P ratio in the bariatric group is indicative of microbiome dysbiosis. In addition, periodontitis + caries caused a more significant shift in microbial diversity. Moreover, microbiome analysis showed a distinct cluster of the bariatric group. Our results suggest that oral microbiome and oral diseases are affected by obesity and weight loss.