IADR Abstract Archives
Prolonged Inhibition of S. Mutans Growth and Biofilm Formation by Sustained Release of Chlorhexidine, Coating Dental Abutments
Objectives: To evaluate the anti-bacterial/anti-biofilm effect of chlorhexidine incorporated into sustained- release varnish (SRV-CHX) coating on dental abutments
Methods: Three kinds of dental abutments: PEAK healing abutment (1), Titanium healing abutment (2) and Titanium permanent abutment (3) were coated with SRV-CHX or SRV-Placebo. SRV-CHX/Placebo- coated abutments were placed on pre-seeded with S. mutans BHI agar plates with following 24 h incubation. SRV-CHX/Placebo -coated abutments were transferred each day to the new pre-seeded BHI plate for a period for 36 days. Effect of SRV-CHX on S. mutans growth on agar plates was detected by measuring the zone of inhibition (ZOI) around each tested abutment each day. Biofilm formation on the SRV-CHX/Placebo coated abutments was detected using CLSM and SEM after 7 and 13 days of abutments transferring, respectively. Well-associated biofilms formed around SRV-CHX/Placebo-coated abutments were monitored by transferring abutments each day to the new well with bacterial inoculum supplied with sucrose for 21 days using crystal violet (CV) assay. Remained CHX concentration in SRV-CHX coated abutments was detected after 13 days of abutments transferring using EDX-SEM assay.
Results: All tested SRV-CHX coated abutments demonstrated long-term inhibition of bacterial growth. SRV-CHX coated 2 and 3 were able to inhibit S. mutans growth during 34 days, while 1 inhibited growth during 32 days. All tested SRV-CHX coated abutments exhibited pulsatile kinetic inhibition pattern probably due to pulsatile release of CHX by SRV during the time of incubation. Furthermore, abutment-associated biofilm formation was notably inhibited by SRV-CHX coating after 7 and 13 days of incubation with S. mutans. Finally, biofilm formed around SRV-CHX -coated abutments was totally inhibited up to 12 days of abutments transferring. Interestingly, that some amount (1.8%-4.6%) CHX remained in SRV coating after 13 days.
Conclusions: Coating of dental abutments with SRV-CHX demonstrated long-term effective inhibition of cariogenic bacteria growth and biofilm formation on the abutment surface as well as around the denture devise. Therefore, SRV-CHX -coated abutments could be a promising strategy for the prevention of dental implant-associated infections.
Methods: Three kinds of dental abutments: PEAK healing abutment (1), Titanium healing abutment (2) and Titanium permanent abutment (3) were coated with SRV-CHX or SRV-Placebo. SRV-CHX/Placebo- coated abutments were placed on pre-seeded with S. mutans BHI agar plates with following 24 h incubation. SRV-CHX/Placebo -coated abutments were transferred each day to the new pre-seeded BHI plate for a period for 36 days. Effect of SRV-CHX on S. mutans growth on agar plates was detected by measuring the zone of inhibition (ZOI) around each tested abutment each day. Biofilm formation on the SRV-CHX/Placebo coated abutments was detected using CLSM and SEM after 7 and 13 days of abutments transferring, respectively. Well-associated biofilms formed around SRV-CHX/Placebo-coated abutments were monitored by transferring abutments each day to the new well with bacterial inoculum supplied with sucrose for 21 days using crystal violet (CV) assay. Remained CHX concentration in SRV-CHX coated abutments was detected after 13 days of abutments transferring using EDX-SEM assay.
Results: All tested SRV-CHX coated abutments demonstrated long-term inhibition of bacterial growth. SRV-CHX coated 2 and 3 were able to inhibit S. mutans growth during 34 days, while 1 inhibited growth during 32 days. All tested SRV-CHX coated abutments exhibited pulsatile kinetic inhibition pattern probably due to pulsatile release of CHX by SRV during the time of incubation. Furthermore, abutment-associated biofilm formation was notably inhibited by SRV-CHX coating after 7 and 13 days of incubation with S. mutans. Finally, biofilm formed around SRV-CHX -coated abutments was totally inhibited up to 12 days of abutments transferring. Interestingly, that some amount (1.8%-4.6%) CHX remained in SRV coating after 13 days.
Conclusions: Coating of dental abutments with SRV-CHX demonstrated long-term effective inhibition of cariogenic bacteria growth and biofilm formation on the abutment surface as well as around the denture devise. Therefore, SRV-CHX -coated abutments could be a promising strategy for the prevention of dental implant-associated infections.