IADR Abstract Archives
Ossix Volumax Collagen Scaffold Characterization by Cell Proliferation and Differentiation
Objectives: Bone and soft tissue defects are common pathology in the oral cavity and the craniofacial region Ossix Volumax is a collagen-based porous resorbable, biocompatible scaffold of 1-2 mm thickness. It was engineered for bone and tissue regeneration process allowing adequate bone augmentation and soft tissue healing of craniofacial defects. The aim of this study is to characterize Ossix Volumax cross-link type I collagen scaffold seeded with human bone marrow mesenchymal stem cell and PDL(periodontal ligament) fibroblast cells derived from human extracted teeth, regarding cells proliferation, differentiation, osteogenesis process.
Methods: Ossix Volumax collagen scaffold is a 3D spongy structure fabricated from cross linked type I collagen fibers extracted and purified from porcine tendons (provided by Datum Dental, Israel). Bone marrow mesenchymal stem cell hBM-MSC were cultured on the collagen scaffold in osteogenic induction media for 21 days. Osteobalst differentiation tested by Alkaline phosphatase activity. PDL's fibrolasts derived from extracted teeth, were cultured on the scaffold in proliferation media for 0,3,7,10, 14 days. The proliferative potential of these cells on the collagen scaffold was analyzed by confocal laser scanning microscopy.
Results: Alkaline phosphatase activity staining demonstrated the differentiation ability of Mesenchymal Stem Cells (MSC) seeded on the scaffold in osteogenic induction media towards osteoblasts activity. Confocal laser scanning microscopy revealed the preservation of the unique fibroblast cells structure on the collagen scaffold and the increase in cell number along time frame.
Conclusions: Oossix Volumax is a novel 3D, collagen scaffold. It presents an effective biomedical platform for bone and soft tissue regeneration of for the treatment of craniofacial bone defects.
Methods: Ossix Volumax collagen scaffold is a 3D spongy structure fabricated from cross linked type I collagen fibers extracted and purified from porcine tendons (provided by Datum Dental, Israel). Bone marrow mesenchymal stem cell hBM-MSC were cultured on the collagen scaffold in osteogenic induction media for 21 days. Osteobalst differentiation tested by Alkaline phosphatase activity. PDL's fibrolasts derived from extracted teeth, were cultured on the scaffold in proliferation media for 0,3,7,10, 14 days. The proliferative potential of these cells on the collagen scaffold was analyzed by confocal laser scanning microscopy.
Results: Alkaline phosphatase activity staining demonstrated the differentiation ability of Mesenchymal Stem Cells (MSC) seeded on the scaffold in osteogenic induction media towards osteoblasts activity. Confocal laser scanning microscopy revealed the preservation of the unique fibroblast cells structure on the collagen scaffold and the increase in cell number along time frame.
Conclusions: Oossix Volumax is a novel 3D, collagen scaffold. It presents an effective biomedical platform for bone and soft tissue regeneration of for the treatment of craniofacial bone defects.