IADR Abstract Archives
Autologous adipose tissue derived MSCs transplantation promote bone regeneration in maxillary bone cleft defect transplantation
Objectives:
Stem cell therapy is a groundbreaking treatment of diseases and injury, also involved congenital and acquired bone defects of the jaws.
The aim of the current study is to evaluate the implantation potential of mesenchimal stem cells (MSCs) originated from adipose tissue into a critical-size alveolar cleft defect in regeneration of new bone tissue in a rat model.
Methods:
44 rates were participated in the study. After the performance of bony cleft defects rates were divided into 3 groups: : 1. groups included alveolar cleft negative control (Defect only), 2. mineralized cortical bone scaffold and3. mineralized cortical bone scaffold seeded with MSCs originated from adipose tissue. The rats' implanted defect subjected to µCT scan folowed by histological stains (H&E, Masson Tri chrom and Picro Sirious Red) and immune histological analysis (Ostrix and HNA).
Results:
New bone formation was calculated in the defect area after 6\8 weeks. A Mann-Whitney test was applied to determine significant intergroup differences. Analysis with microcomputed tomography showed formation of new bone in the defect area but not in a prominent way in comparison to that of the cell-free scaffold group. Histological and Immune histological analysis did not show new bone formation.
Conclusions:
Adipose tissue derived MSCs seeded on a mineral scaffold may present bone regenerated potantial in maxillary defect in rat that immitate cleft plate. Further research is required to examine the ability of these cells on bone formation. Taking into further consideration the use of wild type animal model, increasing the number of tested animals and examining the period until sacrifice may improve results.
This study is expending now days in creating a similar model for large animals.
Stem cell therapy is a groundbreaking treatment of diseases and injury, also involved congenital and acquired bone defects of the jaws.
The aim of the current study is to evaluate the implantation potential of mesenchimal stem cells (MSCs) originated from adipose tissue into a critical-size alveolar cleft defect in regeneration of new bone tissue in a rat model.
Methods:
44 rates were participated in the study. After the performance of bony cleft defects rates were divided into 3 groups: : 1. groups included alveolar cleft negative control (Defect only), 2. mineralized cortical bone scaffold and3. mineralized cortical bone scaffold seeded with MSCs originated from adipose tissue. The rats' implanted defect subjected to µCT scan folowed by histological stains (H&E, Masson Tri chrom and Picro Sirious Red) and immune histological analysis (Ostrix and HNA).
Results:
New bone formation was calculated in the defect area after 6\8 weeks. A Mann-Whitney test was applied to determine significant intergroup differences. Analysis with microcomputed tomography showed formation of new bone in the defect area but not in a prominent way in comparison to that of the cell-free scaffold group. Histological and Immune histological analysis did not show new bone formation.
Conclusions:
Adipose tissue derived MSCs seeded on a mineral scaffold may present bone regenerated potantial in maxillary defect in rat that immitate cleft plate. Further research is required to examine the ability of these cells on bone formation. Taking into further consideration the use of wild type animal model, increasing the number of tested animals and examining the period until sacrifice may improve results.
This study is expending now days in creating a similar model for large animals.