IADR Abstract Archives
RT-PCR for testing the effect of blue light on multi-species biofilm
Objectives: Introduction: The high biocompatibility of titanium in human tissue has favored its use as an implant material for the replacement of missing teeth. However, bacterial accumulation into biofilm on dental implant surfaces induces inflammatory changes of the implant surrounding tissues , that may lead to their progressive destruction by peri-implantitis and ultimately to implant failure. Surface characteristics of implants complicate the conventional treatments, thus alternative antimicrobial approaches using light treatment have been suggested.
Objectives: To examine the effect of blue light treatment on bacterial population of multi- species biofilm grown on titanium surfaces in vitro.
Methods: Materials and Methods: Multi- species biofilm composed of four oral bacteria: Streptococcus sanguis, Actinomyces naeslundii, Fusobacterium nucleatum, and Porphyromonas gingivalis, were grown on titanium or hydroxyapatite surfaces and exposed to blue light using fluence of 37 j/cm2. The four bacterial species were quantified 24h after light treatment using Real Time PCR. Biofilm structure and bacterial viability were examined 24h after light treatment by using the Confocal Scanning Laser Microscopy (CSLM) with live/dead staining.
Results: Results: RT-PCR analysis showed a significant decrease in the quantity of F.nucleatum and P.gingivalis 24h after blue light treatment. This effect was observed both on the titanium and the hydroxyapatite surfaces. On the other hand, S.sanguis and A.naeslundii were increase 24h after blue light treatment. The live/dead analysis after using the CSLM was not yet performed.
Conclusions: Conclusion: These results indicate a possible selective susceptibility to blue light treatment of bacteria in biofilm accumulated on titanium surfaces. Blue light may be used for targeting specific periodontal pathogens, thus, potentially altering the equilibrium inside the biofilm.
Objectives: To examine the effect of blue light treatment on bacterial population of multi- species biofilm grown on titanium surfaces in vitro.
Methods: Materials and Methods: Multi- species biofilm composed of four oral bacteria: Streptococcus sanguis, Actinomyces naeslundii, Fusobacterium nucleatum, and Porphyromonas gingivalis, were grown on titanium or hydroxyapatite surfaces and exposed to blue light using fluence of 37 j/cm2. The four bacterial species were quantified 24h after light treatment using Real Time PCR. Biofilm structure and bacterial viability were examined 24h after light treatment by using the Confocal Scanning Laser Microscopy (CSLM) with live/dead staining.
Results: Results: RT-PCR analysis showed a significant decrease in the quantity of F.nucleatum and P.gingivalis 24h after blue light treatment. This effect was observed both on the titanium and the hydroxyapatite surfaces. On the other hand, S.sanguis and A.naeslundii were increase 24h after blue light treatment. The live/dead analysis after using the CSLM was not yet performed.
Conclusions: Conclusion: These results indicate a possible selective susceptibility to blue light treatment of bacteria in biofilm accumulated on titanium surfaces. Blue light may be used for targeting specific periodontal pathogens, thus, potentially altering the equilibrium inside the biofilm.