IADR Abstract Archives
Cariogenic potential of infant formulas
Objectives: To compare the in vitro cariogenic potential different kinds of IF and to test the in vitro cariogenic potential of IF based on fluoridated water.
Methods: Two brands of IF suitable to infants aged 6-12 months were selected: 'Materna Extra Care' stage 2 and 'Similac Advance Plus' stage 2. The formulas were prepared according to the manufacturer’s instructions in two methods: 1. based on sterile distilled water. 2. based on sterile distilled water containing Fluoride (1 ppm). In each group, 3 specimens were inoculated with Streptococcus mutans (SM) suspension. All specimens were incubated at 37 degrees C for 4 hours in anaerobic conditions. Six-fold dilutions of each sample were spread onto individual MS agar plates in triplicates that were incubated under anaerobic conditions following which, colony forming units (CFUs) of SM was enumerated. The pH changes associated with bacterial fermentation were assessed by sampling each of the experimental and control suspensions for pH measurement at time 0, and 4 hours following incubation, using pH meter.
Results: The pH was lower at T4 than T0 in both formulas. A statistically significant increase in SM colonies at T4 compared to T0 was found in both formulas. There was no statistically difference between the CFUs of the mediums at T0 and T4 of both IF. No statistically significant relation was found in SM colonies in IF based on sterile distilled fluoridated water in comparison to distilled water alone.
Conclusions: The study did not find a significant difference in cariogenic potential between the two infant formulas that were tested.
Methods: Two brands of IF suitable to infants aged 6-12 months were selected: 'Materna Extra Care' stage 2 and 'Similac Advance Plus' stage 2. The formulas were prepared according to the manufacturer’s instructions in two methods: 1. based on sterile distilled water. 2. based on sterile distilled water containing Fluoride (1 ppm). In each group, 3 specimens were inoculated with Streptococcus mutans (SM) suspension. All specimens were incubated at 37 degrees C for 4 hours in anaerobic conditions. Six-fold dilutions of each sample were spread onto individual MS agar plates in triplicates that were incubated under anaerobic conditions following which, colony forming units (CFUs) of SM was enumerated. The pH changes associated with bacterial fermentation were assessed by sampling each of the experimental and control suspensions for pH measurement at time 0, and 4 hours following incubation, using pH meter.
Results: The pH was lower at T4 than T0 in both formulas. A statistically significant increase in SM colonies at T4 compared to T0 was found in both formulas. There was no statistically difference between the CFUs of the mediums at T0 and T4 of both IF. No statistically significant relation was found in SM colonies in IF based on sterile distilled fluoridated water in comparison to distilled water alone.
Conclusions: The study did not find a significant difference in cariogenic potential between the two infant formulas that were tested.