IADR Abstract Archives
Selective Effect of Titanium Surface Treatement on Multi Species Biofilm and Host Cells
Objectives: Peri-implantitis may lead to dental implant failure with loss of supporting bone. The cause of peri-implant tissue breakdown is multifactorial, but bacterial infection and host immune cell reaction are considered to be major factors. Here we examine the effect of different titanium surface modifications on multi species biofilm formation and host cells.
Methods: Mixed bacterial biofilm consisting of four strains: S.sanguis, A.naeslundii, P.gingivalis and F.nucleatum, were grown on different titanium surface covering (machine-surfaced, sand-blasted, SLA-etched & ZnCuO-processed) in 96-well culture plate. Biofilm’s bacteria grown on different titanium surfaces were quantified using selective cultures for viable count test, and by RT-PCR. Biofilm structure and bacterial viability were examined by using the Confocal Scanning Laser Microscopy (CSLM) with live/dead stain. RAW 264.7 macrophage cell lines and Human osteoblast-like cells SaOS-2 were grown on the modified surfaces above. The host cells were quantified using XTT cell proliferation assay, and cellular activation will evaluated later by TNF-alpha assay (for macrophages) and Alkaline phosphatase (ALP) assay (for osteoblasts).
Results: After incubation in anaerobic conditions for 14 days, all biofilm species were grown on all titanium surfaces and identified successfully by light microscopy. DNA was purified and examined by rt-PCR. ZnCuO-processed titanium surface found to decrease biofilm growth by more than 30% comparing with controls. SLA-etched titanium was less favorable for biofilm growth comparing with sand-blasted titanium. These findings were approved also by CSLM. We observed an increase in macrophages vitality on sand-blasted titanium surface compared with acid-etched surface; conversely, Osteoblasts show increased growth on SLA-etched surface.
Conclusions: Our results demonstrate that topographical control using a different titanium surface covering has affected both host cells vitality and biofilm growth. Taken together, our findings lead to a suggestion that the implant topography can play a pivotal role in inflammation regulation and implants biocompatibility.
Methods: Mixed bacterial biofilm consisting of four strains: S.sanguis, A.naeslundii, P.gingivalis and F.nucleatum, were grown on different titanium surface covering (machine-surfaced, sand-blasted, SLA-etched & ZnCuO-processed) in 96-well culture plate. Biofilm’s bacteria grown on different titanium surfaces were quantified using selective cultures for viable count test, and by RT-PCR. Biofilm structure and bacterial viability were examined by using the Confocal Scanning Laser Microscopy (CSLM) with live/dead stain. RAW 264.7 macrophage cell lines and Human osteoblast-like cells SaOS-2 were grown on the modified surfaces above. The host cells were quantified using XTT cell proliferation assay, and cellular activation will evaluated later by TNF-alpha assay (for macrophages) and Alkaline phosphatase (ALP) assay (for osteoblasts).
Results: After incubation in anaerobic conditions for 14 days, all biofilm species were grown on all titanium surfaces and identified successfully by light microscopy. DNA was purified and examined by rt-PCR. ZnCuO-processed titanium surface found to decrease biofilm growth by more than 30% comparing with controls. SLA-etched titanium was less favorable for biofilm growth comparing with sand-blasted titanium. These findings were approved also by CSLM. We observed an increase in macrophages vitality on sand-blasted titanium surface compared with acid-etched surface; conversely, Osteoblasts show increased growth on SLA-etched surface.
Conclusions: Our results demonstrate that topographical control using a different titanium surface covering has affected both host cells vitality and biofilm growth. Taken together, our findings lead to a suggestion that the implant topography can play a pivotal role in inflammation regulation and implants biocompatibility.