IADR Abstract Archives
Bacterial induced experimental per-implantitis in mice
Objectives: The aim of this study was to develop a murine model for bacteria-induced alveolar bone loss around titanium implants and to study the alveolar bone loss around implant in various conditions compared to the alveolar bone loss around teeth
Methods: Screw-shaped titanium implants, smooth surface or SLA coated, were inserted immediately after extraction of the first upper left molar, in 5-6-week-old BALB/c mice. The mice were infected with Porphyromonas gingivalis and Fusobacterium nucleatum 21 (immediate infection) or 42 days (delayed infection) after implantation. Six weeks post infection, bone volume around the inserted implants was measured using micro-CT, and was compared to alveolar bone level around teeth. Histological analysis was performed
Results: The bacterial infection induced alveolar bone loss around the teeth and the implants. We noticed a difference in the survival rate of the immediately infected implant (60%) compared to the delayed in infected group (100%). Through micro CT analysis, we found that the level of bone loss was significantly higher around the implants compared to the teeth, for both SLA coated implants and smooth surface implants. The level of bone loss around the smooth surface implants was higher than the SLA surface in both control and infected groups with no statistical significance. There is no significant difference between the early and the delayed infection in alveolar bone loss level around the implants
Conclusions: The results show that the alveolar bone resorption around implants is higher compared to teeth, which may indicate a more severe infection reaction around implants by the same pathogens. In addition the results might indicate that a delayed infection method is superior for a model of osseointegration and induced periimplantitis. This research will open new possibilities towards studying peri-implantitis, and understanding its pathogenesis
Methods: Screw-shaped titanium implants, smooth surface or SLA coated, were inserted immediately after extraction of the first upper left molar, in 5-6-week-old BALB/c mice. The mice were infected with Porphyromonas gingivalis and Fusobacterium nucleatum 21 (immediate infection) or 42 days (delayed infection) after implantation. Six weeks post infection, bone volume around the inserted implants was measured using micro-CT, and was compared to alveolar bone level around teeth. Histological analysis was performed
Results: The bacterial infection induced alveolar bone loss around the teeth and the implants. We noticed a difference in the survival rate of the immediately infected implant (60%) compared to the delayed in infected group (100%). Through micro CT analysis, we found that the level of bone loss was significantly higher around the implants compared to the teeth, for both SLA coated implants and smooth surface implants. The level of bone loss around the smooth surface implants was higher than the SLA surface in both control and infected groups with no statistical significance. There is no significant difference between the early and the delayed infection in alveolar bone loss level around the implants
Conclusions: The results show that the alveolar bone resorption around implants is higher compared to teeth, which may indicate a more severe infection reaction around implants by the same pathogens. In addition the results might indicate that a delayed infection method is superior for a model of osseointegration and induced periimplantitis. This research will open new possibilities towards studying peri-implantitis, and understanding its pathogenesis