IADR Abstract Archives
Cellular and extracellular organization analysis of Peri-Implantitis (PI) lesions
Objectives: Histological analyses of composition and distribution of cellular and extracellular matrix components of peri–implantitis lesions in humans
Methods: The study was conducted as a retrospective study based on biopsies that were extracted from lesions identified as PI between 2014 and 2016. Diagnosis of PI was done using clinical signs (erythema, swelling, bleeding on probing and pocket formation with or without suppuration) and radiological evidence of cervical bone loss. Periodontal lesions served as control. Detailed histological study included 118 biopsies (study 92 and 26 controls) retrieved from 97 patients. Following biopsy, tissue samples were placed in formalin and paraffin embedded. Sections were prepared for histological and immunohistochemical analysis. Clinical and radiological data were also obtained from all implants of study population (770 implants, infected and healthy).
Results: A well-defined keratinocyte epithelium was present on the top of all the study group specimens; under this epithelium a wide inflammatory cell infiltrates (ICT) was present and for about 60% represented by plasma cells. Collagen type I fibers were present in the form of parallel cords. A different distribution of blood vessels was also present from the apical to the most coronal portion of the specimens.
Conclusions: Our study suggests that peri-implantitis lesions exhibit a well defined biological unique organization not only in term of inflammatory cells but also on epithelial, vessel and extracellular matrix components.
Methods: The study was conducted as a retrospective study based on biopsies that were extracted from lesions identified as PI between 2014 and 2016. Diagnosis of PI was done using clinical signs (erythema, swelling, bleeding on probing and pocket formation with or without suppuration) and radiological evidence of cervical bone loss. Periodontal lesions served as control. Detailed histological study included 118 biopsies (study 92 and 26 controls) retrieved from 97 patients. Following biopsy, tissue samples were placed in formalin and paraffin embedded. Sections were prepared for histological and immunohistochemical analysis. Clinical and radiological data were also obtained from all implants of study population (770 implants, infected and healthy).
Results: A well-defined keratinocyte epithelium was present on the top of all the study group specimens; under this epithelium a wide inflammatory cell infiltrates (ICT) was present and for about 60% represented by plasma cells. Collagen type I fibers were present in the form of parallel cords. A different distribution of blood vessels was also present from the apical to the most coronal portion of the specimens.
Conclusions: Our study suggests that peri-implantitis lesions exhibit a well defined biological unique organization not only in term of inflammatory cells but also on epithelial, vessel and extracellular matrix components.