IADR Abstract Archives
Mixed Streptococcus mutans - streptococcus sanguinis biofilm on dental enamel
Objectives: The dental biofilm is the leading etiological cause of dental caries, gingivitis and periodontal diseases. Dental caries pathogenesis involves the cariogenic bacteria Streptococcus mutans and Streptococcus sanguinis. Our aim was to develop a dual S. mutans-S. Sanguinis biofilm on dental enamel and to determine the effects of blue light irradiation on the mutual and cooperative interactions in the newly formed dual-biofilm on enamel.
Methods: Biofilm of S. mutans or S. sanguinis or mixed biofilm of both, was grown ex-vivo on bovine enamel. The biofilm was generated in BHI with or without 2% sucrose. The biomass was quantified and characterized using crystal violet staining, CLSM, and SEM. qPCR was performed to quantify the DNA concentration of each bacterial strain on the enamel 24h after treatment with blue LED light (400-500nm) using 112 J/cm2.
Results: The best biofilm formation in both bacterial strains requires 2% sucrose supplementation in order to adhere to the surface. The DNA concentration of S. sanguinis growth on enamel was 87% greater than S. mutans. However, in mixed biofilm, a significant decrease of S. sanguinis by 77% and a slight increase in S. mutans by 28% was measured, in comparison with each species biofilm formation. Moreover, blue light had a significant antibacterial effect on single species and mixed biofilms. Single species biofilms of S. mutans were reduced by 81%, and that of S. sanguinis by 79% as compare to control non-irradiated biofilms. Mixed biofilms of the two bacteria were reduced by 74% of S. sanguinis and 38% of S. mutans as compared to control.
Conclusions: S. mutans and S. sanguinis can serve as a model to investigate the interspecies interactions leading to competition and coexistence on dental biofilm. Our study demonstrates that blue light was able to effectively reduce the biofilm formation on enamel surface which are associated with dental caries.
Methods: Biofilm of S. mutans or S. sanguinis or mixed biofilm of both, was grown ex-vivo on bovine enamel. The biofilm was generated in BHI with or without 2% sucrose. The biomass was quantified and characterized using crystal violet staining, CLSM, and SEM. qPCR was performed to quantify the DNA concentration of each bacterial strain on the enamel 24h after treatment with blue LED light (400-500nm) using 112 J/cm2.
Results: The best biofilm formation in both bacterial strains requires 2% sucrose supplementation in order to adhere to the surface. The DNA concentration of S. sanguinis growth on enamel was 87% greater than S. mutans. However, in mixed biofilm, a significant decrease of S. sanguinis by 77% and a slight increase in S. mutans by 28% was measured, in comparison with each species biofilm formation. Moreover, blue light had a significant antibacterial effect on single species and mixed biofilms. Single species biofilms of S. mutans were reduced by 81%, and that of S. sanguinis by 79% as compare to control non-irradiated biofilms. Mixed biofilms of the two bacteria were reduced by 74% of S. sanguinis and 38% of S. mutans as compared to control.
Conclusions: S. mutans and S. sanguinis can serve as a model to investigate the interspecies interactions leading to competition and coexistence on dental biofilm. Our study demonstrates that blue light was able to effectively reduce the biofilm formation on enamel surface which are associated with dental caries.