Oral squamous cell carcinomas (OSCC) are among the 10 most common cancers in the world with smoking as the major risk factor. Earlier identification of high risk patients for the disease is extremely important for primary prevention and to obtain better local disease control. Aneuploidy, an aberrant chromosome number is the most common characteristic of human cancers, and plays an important role in tumor formation and development. Chromosomal numerical aberrations were found to be an early event in oral carcinogenesis and can be therefore used as a predictive marker.
Objectives:
We aimed to investigate the prevalence of aneuploid cells (ACs) in oral samples obtained from heavy smokers and normal subjects.
Methods:
The study group included 93 heavy smokers, past smokers and second-hand smokers, and 35 normal control subjects. From each case two samples were collected using a disposable brush. The samples were simultaneously analyzed for morphology and fluorescent in-situ hybridization (FISH) using chromosomes 2 and 8 centromeric probes.
Results:
Two of the control subjects had 1% and 1.5% ACs in the examined samples. A cut off value of aneuploid cells (ACs) was determined as 1.1%. ACs were found in 10% of the heavy smokers and in 5% of the second-hand smokers.
Conclusions:
Heavy smoking harbors an increased risk for chromosomal instability. Identifying ACs in a brush sample can increase the specificity in identifying a subgroup of high risk patients that may develop oral cancer.
Division: Israeli Division Meeting
Meeting:2008 Israeli Division Meeting (Tel Aviv, Israel) Location: Tel Aviv, Israel
Year: 2008 Final Presentation ID: Abstract Category|Abstract Category(s):Scientific Groups
Authors
Shani, Tali
( The Maurice and Gabriela Goldschleger School of Dental Medicine, Tel Aviv University, Tel-Aviv, N/A, Israel
)
Cabba, Ala
( The Maurice and Gabriela Goldschleger School of Dental Medicine, Tel Aviv University, Tel-Aviv, N/A, Israel
)
Ben Dov, Issaschar
( The Chaim Sheba Medical Center, Tel-Hashomer, Israel, Tel-Hashomer, N/A, Israel
)
Adam, Iris
( The Chaim Sheba Medical Center, Tel-Hashomer, Israel, Tel-Hashomer, N/A, Israel
)
Amariglio, Ninette
( The Chaim Sheba Medical Center, Tel-Hashomer, Israel, Tel-Hashomer, N/A, Israel
)
Rechavi, Gideon
( The Chaim Sheba Medical Center, Tel-Hashomer, Israel, Tel-Hashomer, N/A, Israel
)
Trakhtenbrot, Luba
( The Chaim Sheba Medical Center, Tel-Hashomer, Israel, Tel-Hashomer, N/A, Israel
)
Hirshberg, Abraham
( The Maurice and Gabriela Goldschleger School of Dental Medicine, Tel Aviv University, Tel-Aviv, N/A, Israel
)