Several attempts to develop reliable tools for caries risk-screening have been made over the years. According to the multicausal aetiology of caries, different approaches have been used: microbiological, biochemical, anamnestic and clinical approaches. In prospective clinical studies, white spot lesions and discoloured fissures on first molars where found to be the most reliable discriminating parameters in 6- to 12-year-old children. Until now the detection and enumeration of mutans streptococci and lactobacilli was not proven to be a reliable screening tool for caries risk. A high concentration of lactobacilli in saliva is more likely to be the result of cavities than its cause. On the other hand the detection of mutans streptococci can provide useful information. For example it was shown that the presence of these bacteria in saliva and plaque of 5-year-old caries free children is associated with an elevated relative risk to get new caries lesions. Even if this finding does not yet allow a reliable discrimination between children at risk and not at risk, it shows that the detection of caries related micro-organisms may go in the right direction. The detection of the right micro-organisms with a reliable method may be a step forward to develop a reliable caries-risk assessment.