Evaluation of TEGDMA leaching from four resin cements by HPLC

Purpose: The commonly used dental base monomers bisphenol A glycol dimethacrylate (Bis-GMA) and urethane dimethacrylate (UDMA) require the use of a diluent monomer, such as triethylene glycol dimethacrylate (TEGDMA). The aim of this study was to evaluate in vitro elution of TEGDMA from dual cured resin cements, used for bonding of ceramic restoration by high performance liquid chromatography (HPLC). Material and methods: Fourty freshly extracted caries and restoration free molar teeth used in this study. Standardized Class I preparations were prepared in all teeth. A lithium disilicate based (IPS Empress Esthetic; Ivoclar) ceramic inlays were cemented with one of the dual cured resin cements (Variolink II; Ivoclar, Rely X ARC; 3M ESPE, Rely X Unicem; 3M ESPE and Resilute; Pulpdent). After cementation, specimens were stored in 75% ethanol solution. HPLC was used to analyze the amounts of TEGDMA in the solution (after 10 min, 1 hour, 24 hours, 3, 7, 14 and 21 days). Two-way ANOVA and Tukey HSD tests were used to evaluate the results (p<.05). Results: There were statistically significant differences among the amount of the monomers (p<.01) and also among time periods (p<.01). The amount of TEGDMA eluted from Resilute was the highest and the amount of TEGDMA eluted from Rely X Unicem was the lowest (p<.01). The total amount of monomers was the highest after 21 days (p<.01). Conclusion: In the case of resin cements, elution of TEGDMA was the highest in Resilute and lowest in Rely X Unicem. The amount of TEGDMA eluted from resin cements was influenced by the time.