IADR Abstract Archives

Survival of Dental Pulp Cells in Vascularized Tissue Engineering Chambers

Background and Objective: Dental pulp stem cells (DPSCs), recognized in both permanent and deciduous dental pulp tissue, can generate pulp-like tissue in vivo (1). Using primary pulp cells (PPCs) expanded in culture, we wish to establish a model for growing dental pulp tissue by seeding PPC into a vascularized tissue engineering chamber model in the rat.

Methods: Single cell suspensions of DiI labeled inbred rat primary pulp cells passage 1 -5 were mixed with collagen scaffold together with or without gelatin microspheres containing BMP-4 and bFGF. The mixture was then added into silicon chambers placed in the rat groin area using a protocol modified from Cronin et al 2004 (2). Chambers were harvested at 2 ,4, 6, and 8 weeks, processed for morphology, histology, and immunohistochemistry.

Results: After 2 week transplantation, new tissues together with new blood vessel formation were observed inside the chamber. Labeled dental pulp cells were found particularly around vascular pedicle and also close to the gelatin microspheres. Cell survival, confirming by double fluorescent staining with DaPI, was reported up to 8 week after transplantation.

Conclusion: Vascularized tissue engineering chamber in the rat may provide a useful model for in vivo study of dental pulp cells and their interactions. Survival of dental pulp cells can be observed in this model up to 8 weeks. Further analysis of this in vivo model will be reported determining whether dental pulp tissue can be generated in this fashion.

This research was supported by Australian Dental Research Foundation (33/2005) and Australian Society of Endodontics.

(1) Gronthos S et al (2000) PNAS 97:13625-30. (2) Cronin KJ et al (2004). Plast Reconstr Surg113:1-10.


Continental European and Israeli Divisions Meeting
2007 Continental European and Israeli Divisions Meeting (Thessaloniki, Greece)
Thessaloniki, Greece
2007
140
Scientific Groups
  • Srisuwan, Tanida  ( Bernard O’Brien Institute of Microsurgery, The University of Melbourne, Melbourne, Victoria, N/A, Australia )
  • Tilkorn, Daniel  ( Bernard O’Brien Institute of Microsurgery, The University of Melbourne, Melbourne, Victoria, N/A, Australia )
  • Abberton, Keren  ( Bernard O’Brien Institute of Microsurgery, The University of Melbourne, Melbourne, Victoria, N/A, Australia )
  • Messer, Harold H.  ( School of Dental Science, The University of Melbourne, Melbourne, Victoria, N/A, Australia )
  • Thompson, Erik  ( Bernard O’Brien Institute of Microsurgery, The University of Melbourne, Melbourne, Victoria, N/A, Australia )
    • Oral Session
    Pulp Biology
    09/27/2007