Disinfection Methods to Control Microbial Adhesion of Candida albicans
Buergers R, Hahnel S, Rosentritt M and Handel G
University Medical Center Regensburg, Department of Prosthetic Dentistry, Germany
Objectives: Candida albicans is the primary aetologic agent of oral candidosis, which is in turn the major factor in the pathogenesis of denture-related stomatitis. The aim of this in-vitro study was to identify the most effective disinfection procedure to reduce candida plaque on soft denture lining materials.
Methods: Round specimens (diameter 8mm, thickness 2mm) were made of a resilient direct lining material (Mucopren E, Kettenbach) and artificially aged in a thermal cycler (3000 cycles 5°C/55°C). Specimens were incubated for 2.5 hours with Candida albicans (ATCC 10231), followed by one of 9 disinfection protocols (see results). Incubation with phosphate buffered saline (PBS) served as control. Adherent viable C. albicans was quantified using a luminescence kit (ViaLiaght, Cambrex, US) and an automated platereader (Fluostar Optima, bmg). High luminescence values corresponded to high number of adherent C. albicans. Statistics: Median (25/75), Mann-Whitney U-test (α = 0.05).
Results:
|
Disinfection method, application time |
Luminescence median; 25%75%) |
|
Hydrogen peroxide 3%, 10 min |
209 (98/269) |
|
Sodium hypochlorite 1%, 10 min |
8 (-19/27) |
|
Glutaraldehyde 2%, 10 min |
136 (35/315) |
|
Household vinegar, 10 min |
407 (185/727) |
|
Listerine coolmint (Pfizer), 10 min |
432 (385/570) |
|
Blend-a-dent 2 Phasen Ultra tabs (Protector & Gamble), 10 min |
51 (25/143) |
|
Microwave irradiation, dry, 580W, 6 min |
307 (202/454) |
|
Microwave irradiation, in 200ml water, 580W, 6 min |
4 (-4/13) |
|
Air drying, 5 hrs |
229 (112/299) |
|
PBS, 10 min (control) |
277 (140/395) |
The medians of the relative luminescence varied between 4 and 432. Compared to the other disinfection procedures, significantly lowest amounts of viable C. albicans were found after disinfection with sodium hypochlorite, Blend-a-dent 2 Phasen Ultra or microwave irradiation immersed in water.
Conclusion: Only sodium hypochlorite, Blend-a-dent 2 Phasen tabs and microwave irradiation immersed in water seemed to be effective in reducing adhesion of C. albicans on soft denture lining materials.