Objectives: PepGen P-15®, a synthetic peptide analogue of collagen, added to bovine bone mineral to produce an enhanced bone graft material. Two experimental defects, periodontal and intrabony, were examined morphometrically to evaluate the re-institution of the surgically deprived tissues when using this biomaterial. Methods: In 9 dogs, two circular defects, 5 mm in diameter and 2 mm in depth, were made on the labial aspect of the maxillary canines, bilaterally: a fenestrated periodontal on the canine root and an intrabony in the alveolar diastema anteriorly. The biomaterial, in putty form, filled both defects on one side; the contralateral side was blood filled (control). Surgical sites were covered with an absorbable membrane. At 4 months, tissue blocks were made using the cutting/grinding non-decalcification method followed by morphometric analysis. In the periodontal fenestration root surface, linear percentage of new cementum (%NC), area percentage of bone (%NB), and residual biomaterial particles (%PEP) were calculated. At the intrabony sites, these same measurements were calculated, except for cementum. Osteoconductivity level (OL) was assessed by measuring the amount of direct NB-to-PEP contact. Correlation test was used to evaluate any significant relationships between the different measured parameters. Results: In the grafted and non-grafted fenestration root surface defects, %NC averaged 59.5% and 73.9% (p<0.05), respectively; %NB averaged 36.1% and 31.4%, respectively; and %PEP averaged 20.6%. The mean percentage of OL was 52.4%. In the intrabony grafted and non-grafted sites, %NB averaged 50.7% and 60.1%, respectively (p<0.05). Residual %PEP averaged 26.1% and OL 35.6%. In between the two different defect types, more %NB and higher %OL were found at the intrabony sites (p< 0.05, for both). Conclusion: This biomaterial possessed high osteoconduction mechanisms. However, at 4-months, a higher tissue regeneration of new cementum and new bone formation were detected at the non-grafted membrane-protected sites.