IADR Abstract Archives
Development of an optimised collagen-based scaffold for dental pulp regeneration
Objectives:
Dental pulp regeneration is a key goal in the field of endodontic dentistry. Most previous attempts to regenerate vital pulp tissue have failed to produce dentine-like tissue in vivo. An innovative approach is needed to create an optimal environment for these cells, enabling successful dental pulp regeneration. Here, we report promising data showing the regenerative capacity of dental pulp stem cells (DPSCs) on an optimized Collagen scaffold. To optimize collagen-based scaffolds for DPSC-based dental pulp regeneration.
Methods:
DPSC behavior on a range of ECM components first was assessed in 2D. Then, primary ECM candidates were used to fabricate 3D scaffolds (in various combinations) of Col I, Collagen III (Col III), Collagen IV (Col IV), and Hyaluronic Acid (HyA). Scaffolds were mechanically/microstructurally characterized and cell behaviour (adhesion, growth and survival) of DPSCs on 3D scaffolds was assessed.
Results:
DPSCs performed best on (proliferation/survival) either on Col I substrates or a combination of Col III and Col I. However, in 3D, DPSCs showed the best responses on scaffolds with a different composition of Col III and Col I and also on pure Collagen III. Moreover, DPSCs in 3D had well-organized architecture resembling connective-like tissue. Scaffold stiffness was in the range of 1kPa across all groups and had an average pore size of 95.5μm.
Conclusions:
While Col I was the optimal substrate for DPSCs in a 2D microenvironment, in 3D an increased concentration of Col III (an important component of dental pulp) in scaffolds resulted in an improved biological response. Scaffolds of either Col III only or a combination of Col I and Col III may be a superior material for pulp tissue regeneration.
Dental pulp regeneration is a key goal in the field of endodontic dentistry. Most previous attempts to regenerate vital pulp tissue have failed to produce dentine-like tissue in vivo. An innovative approach is needed to create an optimal environment for these cells, enabling successful dental pulp regeneration. Here, we report promising data showing the regenerative capacity of dental pulp stem cells (DPSCs) on an optimized Collagen scaffold. To optimize collagen-based scaffolds for DPSC-based dental pulp regeneration.
Methods:
DPSC behavior on a range of ECM components first was assessed in 2D. Then, primary ECM candidates were used to fabricate 3D scaffolds (in various combinations) of Col I, Collagen III (Col III), Collagen IV (Col IV), and Hyaluronic Acid (HyA). Scaffolds were mechanically/microstructurally characterized and cell behaviour (adhesion, growth and survival) of DPSCs on 3D scaffolds was assessed.
Results:
DPSCs performed best on (proliferation/survival) either on Col I substrates or a combination of Col III and Col I. However, in 3D, DPSCs showed the best responses on scaffolds with a different composition of Col III and Col I and also on pure Collagen III. Moreover, DPSCs in 3D had well-organized architecture resembling connective-like tissue. Scaffold stiffness was in the range of 1kPa across all groups and had an average pore size of 95.5μm.
Conclusions:
While Col I was the optimal substrate for DPSCs in a 2D microenvironment, in 3D an increased concentration of Col III (an important component of dental pulp) in scaffolds resulted in an improved biological response. Scaffolds of either Col III only or a combination of Col I and Col III may be a superior material for pulp tissue regeneration.