Processing of Nerve Conduction Channel with Biomimetic Concept from Animal Diaphysis Bone Lesions for Regeneration of Inferior Alveolar Nerve

Abstract: Introduction: Repair is somewhat possible in the peripheral nervous system, resulting from the interaction of cells and the extracellular matrix. Due to the position of the inferior alveolar nerve (IAN) in the trunk of the mandible, various factors can cause damage to IAN. This project aimed to prepare biomimetic neural conduits from the diaphysis bone of rats for healing damaged IAN. Methods: formalin solution to remove soft tissues around the bone and sodium hypochlorite for 2 hours and drying the bones at 50 degrees Celsius for 10 minutes was used, the fat removal was performed by benzene and ethyl alcohol for 3 hours. the 5% hydrogen peroxide solution was used for 7 hours. Cell removal steps were completed by slow and rapid freezing and thawing, followed by treatment with 25% trypsin and treatment with sodium dodecyl sulfate 2%. For the evaluation of the biocompatibility of processed bones, toxicity tests and MTT tests were used. Results: tests showed that the Haversian canal has no toxicity and the cells are attached to the walls of the canals. Conclusion: This study shows that natural bone material after Decontamination and calcium deposition can be a good method for more Studies on biomimetic nerve conduction channels.