Antibiofilm Activity of Silver Nanoparticles Nystatin Conjugates in Candida Species

Background and objectives: Oropharyngeal candidiasis is a common fungal infection in the oral cavity caused by Candida spp. The disease generates irritating infections accompanied by several symptoms like angular cheilitis, thrush (pseudomembranous), and erythematous. The treatment of oral candidiasis requires a long time and is associated with high rates of recurrence. Antifungal medicines which are conjugated with silver (Ag-NPs) nanoparticle is the possible platforms for the reduction of drug resistance. Materials and Methods: In the present study, we reported the invitro antibiofilm activity of nystatin (NYT)-NPS conjugates (Ag) against resistant Candida species isolated in hematological malignancy patients with MTT assay. Results: MTT quantitative results revealed that the ten out of fifteen resistant isolates studied able to form biofilms in vitro. NYT-AG conjugates were more effective than Ag or NYT nanoparticle alone. Conclusion: The present study concluded that NYT-AG conjugates have an inhibitory effect on the biofilm of resistant Candida species isolated from oral cavity of hematological malignancy patients. Further studies are needed to compare efficacy of another nanoparticles in combination with NYT.